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PMID: 1314161 Published · ppublish English Comparative Study Journal Article

Complementation of the cs dis2-11 cell cycle mutant of Schizosaccharomyces pombe by a protein phosphatase from Arabidopsis thaliana.

The EMBO journal ·Vol. 11 ·No. 4 ·1992-04-00 ·Pages 1327-33

Nitschke K, Fleig U, Schell J, Palme K

Abstract

The activities of type I protein phosphatases play a central role in eukaryotic cell cycle control. Here, we report the cloning and characterization from the flowering plant Arabidopsis thaliana of a cDNA clone named PP1-At which is highly homologous to protein phosphatase 1. The deduced amino acid sequence of PP1-At shows that the PP1-At protein is 318 amino acid residues long and has a molecular weight of 35,298 Da. The PP1-At protein has strong similarity to all other known protein phosphatase type 1 catalytic subunits. Approximately 62% of the amino acids are identical to type 1 protein phosphatases of rabbit, mouse, Saccharomyces cerevisiae and Schizosaccharomyces pombe. RNA blot analysis revealed a single mRNA species of approximately the same size as the cDNA isolated. The PP1-At-encoded mRNA of 1.3 kb is abundant in most vegetative Arabidopsis tissues, with the lowest level of expression in leaves. When transferred to the fission yeast S.pombe, the PP1-At-encoded protein can rescue a semidominant mutant, cold sensitive (cs) dis2-11, which under nonpermissive conditions is unable to complete chromosome disjunction.

Related Genes
dis2-11
MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Cycle/genetics Cloning, Molecular Cold Temperature DNA/genetics,isolation & purification Genes, Dominant Genes, Plant Genetic Complementation Test Mice Molecular Sequence Data Mutation Oligodeoxyribonucleotides Phosphoprotein Phosphatases/genetics,metabolism Plants/enzymology,genetics Polymerase Chain Reaction/methods Protein Conformation Protein Phosphatase 1 Rabbits Restriction Mapping Schizosaccharomyces/cytology,genetics Sequence Homology, Nucleic Acid
Chemicals
Oligodeoxyribonucleotides DNA Phosphoprotein Phosphatases Protein Phosphatase 1
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Nitschke K
Max-Planck-Institut für Züchtungsforschung, Köln, FRG.
Fleig U
Schell J
Palme K
References (41)
41 references, click to expand
  1. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  2. Phosphoprotein Phosphatase of Soybean Hypocotyls: PURIFICATION, PROPERTIES, AND SUBSTRATE SPECIFICITIES .
    Plant Physiol. 1980 Sep;66(3):368-74 PMID: 16661438
  3. Molecular genetic analysis of fission yeast Schizosaccharomyces pombe.
    Methods Enzymol. 1991;194:795-823 PMID: 2005825
  4. Okadaic acid: a new probe for the study of cellular regulation.
    Trends Biochem Sci. 1990 Mar;15(3):98-102 PMID: 2158158
  5. Cyanobacterial microcystin-LR is a potent and specific inhibitor of protein phosphatases 1 and 2A from both mammals and higher plants.
    FEBS Lett. 1990 May 21;264(2):187-92 PMID: 2162782
  6. One of the protein phosphatase 1 isoenzymes in Drosophila is essential for mitosis.
    Cell. 1990 Oct 5;63(1):33-46 PMID: 2170019
  7. Distinct, essential roles of type 1 and 2A protein phosphatases in the control of the fission yeast cell division cycle.
    Cell. 1990 Oct 19;63(2):405-15 PMID: 2170029
  8. Protein phosphatase 1 activity in Drosophila mutants with abnormalities in mitosis and chromosome condensation.
    FEBS Lett. 1990 Nov 26;275(1-2):39-43 PMID: 2175717
  9. Identification by molecular cloning of two cDNA sequences from the plant Brassica napus which are very similar to mammalian protein phosphatases-1 and -2A.
    FEBS Lett. 1990 Dec 10;276(1-2):156-60 PMID: 2176161
  10. Fungal elicitor triggers rapid, transient, and specific protein phosphorylation in parsley cell suspension cultures.
    J Biol Chem. 1990 Apr 15;265(11):6360-8 PMID: 2318857
  11. Regulation of microtubule dynamics by cdc2 protein kinase in cell-free extracts of Xenopus eggs.
    Nature. 1990 Jan 18;343(6255):233-8 PMID: 2405278
  12. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
    Anal Biochem. 1987 Apr;162(1):156-9 PMID: 2440339
  13. Expression of CD45 alters phosphorylation of the lck-encoded tyrosine protein kinase in murine lymphoma T-cell lines.
    Proc Natl Acad Sci U S A. 1989 Nov;86(22):8959-63 PMID: 2530588
  14. A suppressor of a HIS4 transcriptional defect encodes a protein with homology to the catalytic subunit of protein phosphatases.
    Cell. 1989 Feb 24;56(4):527-37 PMID: 2537149
  15. Involvement of a type 1 protein phosphatase encoded by bws1+ in fission yeast mitotic control.
    Cell. 1989 Jun 16;57(6):1009-16 PMID: 2544292
  16. Putting it on and taking it off: phosphoprotein phosphatase involvement in cell cycle regulation.
    Cell. 1989 Jun 16;57(6):891-3 PMID: 2544293
  17. The bimG gene of Aspergillus nidulans, required for completion of anaphase, encodes a homolog of mammalian phosphoprotein phosphatase 1.
    Cell. 1989 Jun 16;57(6):987-96 PMID: 2544297
  18. The fission yeast dis2+ gene required for chromosome disjoining encodes one of two putative type 1 protein phosphatases.
    Cell. 1989 Jun 16;57(6):997-1007 PMID: 2544298
  19. An improved procedure for identifying and quantitating protein phosphatases in mammalian tissues.
    FEBS Lett. 1989 Jul 3;250(2):596-600 PMID: 2546812
  20. Remarkable similarities between yeast and mammalian protein phosphatases.
    FEBS Lett. 1989 Jul 3;250(2):601-6 PMID: 2546813
  21. The structure and regulation of protein phosphatases.
    Annu Rev Biochem. 1989;58:453-508 PMID: 2549856
  22. Evidence that the leukocyte-common antigen is required for antigen-induced T lymphocyte proliferation.
    Cell. 1989 Sep 22;58(6):1055-65 PMID: 2550143
  23. Cloning and chromosomal localization of Drosophila cDNA encoding the catalytic subunit of protein phosphatase 1 alpha. High conservation between mammalian and insect sequences.
    Eur J Biochem. 1989 Aug 15;183(3):603-10 PMID: 2550221
  24. Identification of high levels of type 1 and type 2A protein phosphatases in higher plants.
    Biochem J. 1989 Aug 15;262(1):335-9 PMID: 2554892
  25. Protein phosphatases come of age.
    J Biol Chem. 1989 Dec 25;264(36):21435-8 PMID: 2557326
  26. The fission yeast cdc2/cdc13/suc1 protein kinase: regulation of catalytic activity and nuclear localization.
    Cell. 1989 Aug 11;58(3):485-97 PMID: 2569363
  27. Effect of manganese ions on the incorporation of dideoxynucleotides by bacteriophage T7 DNA polymerase and Escherichia coli DNA polymerase I.
    Proc Natl Acad Sci U S A. 1989 Jun;86(11):4076-80 PMID: 2657738
  28. p34cdc2 is located in both nucleus and cytoplasm; part is centrosomally associated at G2/M and enters vesicles at anaphase.
    EMBO J. 1989 Dec 20;8(13):3985-95 PMID: 2686978
  29. Purification and characterization of two wheat-embryo protein phosphatases.
    Biochem J. 1988 Apr 15;251(2):357-63 PMID: 2840890
  30. cdc2 is a component of the M phase-specific histone H1 kinase: evidence for identity with MPF.
    Cell. 1988 Oct 21;55(2):371-8 PMID: 2844417
  31. The product of the mei3+ gene, expressed under control of the mating-type locus, induces meiosis and sporulation in fission yeast.
    EMBO J. 1987 Mar;6(3):729-36 PMID: 3034608
  32. A thousand and one protein kinases.
    Cell. 1987 Sep 11;50(6):823-9 PMID: 3113737
  33. Cold-sensitive and caffeine-supersensitive mutants of the Schizosaccharomyces pombe dis genes implicated in sister chromatid separation during mitosis.
    EMBO J. 1988 May;7(5):1465-73 PMID: 3409871
  34. Activation at M-phase of a protein kinase encoded by a starfish homologue of the cell cycle control gene cdc2+.
    Nature. 1988 Sep 15;335(6187):251-4 PMID: 3412486
  35. Histones and their modifications.
    CRC Crit Rev Biochem. 1986;20(2):201-63 PMID: 3519076
  36. Selection of AUG initiation codons differs in plants and animals.
    EMBO J. 1987 Jan;6(1):43-8 PMID: 3556162
  37. Putative polyadenylation signals in nuclear genes of higher plants: a compilation and analysis.
    Nucleic Acids Res. 1987 Dec 10;15(23):9627-40 PMID: 3697078
  38. Chloroplast phosphoproteins. Evidence for a thylakoid-bound phosphoprotein phosphatase.
    Eur J Biochem. 1980 Feb;104(1):85-9 PMID: 6245872
  39. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  40. The nuclear envelope lamina is reversibly depolymerized during mitosis.
    Cell. 1980 Jan;19(1):277-87 PMID: 7357605
  41. Plant protein phosphatases. Subcellular distribution, detection of protein phosphatase 2C and identification of protein phosphatase 2A as the major quinate dehydrogenase phosphatase.
    Biochem J. 1991 Feb 1;273 ( Pt 3):733-8 PMID: 1847622
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-04-00
Pages
1327-33
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556581
Subset
IM
Databases
GENBANK
L12698, L12699, L12700, L12701, L12702, L12703, S87972, X63895, X63896, X64328
External Links
PubMed source PMC Full Text View journal details
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