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PMID: 12743798 Published · ppublish English Journal Article

Molecular cloning and characterization of an SRCAP chromatin remodeling homologue in Toxoplasma gondii.

Parasitology research ·Vol. 90 ·No. 1 ·2003-05-00 ·Pages 1-8

Sullivan WJ, Monroy MA, Bohne W, Nallani KC, Chrivia J, Yaciuk P, Smith CK, Queener SF

Abstract

We have identified and mapped a gene in Toxoplasma gondii that encodes a homologue of SRCAP (Snf2-related CBP activator protein), a member of the SNF/SWI family of chromatin remodeling factors. The genomic locus (TgSRCAP) is present as a single copy and contains 16 introns. The predicted cDNA contains an open reading frame of 8,775 bp and encodes a protein of 2,924 amino acids. We have identified additional SRCAP-like sequences in Apicomplexa for comparison by screening genomic databases. An analysis of SRCAP homologues between species reveals signature features that may be indicative of SRCAP members. Expression of mRNA encoding TgSRCAP is upregulated when tachyzoite (invasive form) parasites are induced to differentiate into bradyzoites (encysted form) in vitro. Recombinant TgSRCAP protein is functionally equivalent to the human homologue, being capable of increasing transcription mediated by CREB.

MeSH Terms
Adenosine Triphosphatases/analysis,chemistry,genetics Amino Acid Sequence Animals CREB-Binding Protein Chromatin/genetics Cloning, Molecular Gene Expression Regulation Genes, Protozoan HeLa Cells Humans Molecular Sequence Data Nuclear Proteins/metabolism RNA, Messenger/genetics Sequence Homology, Amino Acid Toxoplasma/genetics,isolation & purification Trans-Activators/metabolism Transcription, Genetic
Chemicals
Chromatin Nuclear Proteins RNA, Messenger Trans-Activators CREB-Binding Protein CREBBP protein, human Adenosine Triphosphatases SRCAP protein, human
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Sullivan William J
Department of Pharmacology and Toxicology, Room A-527, Indiana University School of Medicine, 635 Barnhill Drive, Indianapolis, IN 46202-5120, USA. wjsulliv@iupui.edu
Monroy M Alexandra
Bohne Wolfgang
Nallani Karuna C
Chrivia John
Yaciuk Peter
Smith Charles K
Queener Sherry F
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Article Info
Journal
Parasitology research
Abbr.
Parasitol Res
ISSN
0932-0113
DOI
10.1007/s00436-002-0814-1
Email
wjsulliv@iupui.edu
Published
2003-05-00
Epub
2003-00-28
Pages
1-8
Language
English
Region
Germany
NLM ID
8703571
Subset
IM
Databases
GENBANK
AY061650
External Links
PubMed source DOI: 10.1007/s00436-002-0814-1 View journal details
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