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PMID: 12734586 Published · ppublish English Journal Article

Yeast Two-Hybrid: State of the Art.

Biological procedures online ·Vol. 2 ·1999-10-04 ·Pages 1-38

Van Criekinge W, Beyaert R

Abstract

Genome projects are approaching completion and are saturating sequence databases. This paper discusses the role of the two-hybrid system as a generator of hypotheses. Apart from this rather exhaustive, financially and labour intensive procedure, more refined functional studies can be undertaken. Indeed, by making hybrids of two-hybrid systems, customised approaches can be developed in order to attack specific function-related problems. For example, one could set-up a "differential" screen by combining a forward and a reverse approach in a three-hybrid set-up. Another very interesting project is the use of peptide libraries in two-hybrid approaches. This could enable the identification of peptides with very high specificity comparable to "real" antibodies. With the technology available, the only limitation is imagination.

Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Van Criekinge Wim
DEVGEN NV. Technologiepark 9, 9052 Zwijnaarde-Gent. Belgium.; Department of Molecular Biology. Flanders Interuniversity Institute for Biotechnology and University of Ghent, B-9000, Ghent. Belgium. wim.vancriekinge@devgen.com
Beyaert Rudi
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Article Info
Journal
Biological procedures online
Abbr.
Biol Proced Online
ISSN
1480-9222
DOI
10.1251/bpo16
Email
wim.vancriekinge@devgen.com
Published
1999-10-04
Pages
1-38
Language
English
Region
England
NLM ID
100963717
PMCID
PMC140126
External Links
PubMed source DOI: 10.1251/bpo16 PMC Full Text View journal details
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