Home LiteratureArticle Details

PMID: 7708506 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A new version of the two-hybrid assay for detection of protein-protein interactions.

Nucleic acids research ·Vol. 23 ·No. 5 ·1995-03-11 ·Pages 876-8

Le Douarin B, Pierrat B, vom Baur E, Chambon P, Losson R

Abstract

暂无摘要

MeSH Terms

Amino Acid Sequence Animals Base Sequence DNA, Complementary DNA-Binding Proteins/metabolism Genes, Reporter Humans Mice Molecular Sequence Data Plasmids Protein Binding Receptors, Estrogen/metabolism Recombinant Fusion Proteins/metabolism Regulatory Sequences, Nucleic Acid

Chemicals

DNA, Complementary DNA-Binding Proteins Receptors, Estrogen Recombinant Fusion Proteins

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Le Douarin B

Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, Collège de France, Illkirch.

Pierrat B

vom Baur E

Chambon P

Losson R

References (16)

16 references, click to expand

Functional analysis of the human estrogen receptor using a phenotypic transactivation assay in yeast.
Gene. 1992 Oct 1;119(2):237-45 PMID: 1398105
Purification, cloning, and RXR identity of the HeLa cell factor with which RAR or TR heterodimerizes to bind target sequences efficiently.
Cell. 1992 Jan 24;68(2):377-95 PMID: 1310259
RARs and RXRs: evidence for two autonomous transactivation functions (AF-1 and AF-2) and heterodimerization in vivo.
EMBO J. 1993 Jun;12(6):2349-60 PMID: 8389696
Elimination of false positives that arise in using the two-hybrid system.
Biotechniques. 1993 Jun;14(6):920-4 PMID: 8333960
Mammalian Ras interacts directly with the serine/threonine kinase Raf.
Cell. 1993 Jul 16;74(1):205-14 PMID: 8334704
The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases.
Cell. 1993 Nov 19;75(4):805-16 PMID: 8242751
Production and characterization of monoclonal antibodies recognising defined regions of the human oestrogen receptor.
Hybridoma. 1993 Aug;12(4):391-405 PMID: 7503939
Isolation of the Rb-related p130 through its interaction with CDK2 and cyclins.
Genes Dev. 1993 Dec;7(12A):2378-91 PMID: 8253384
The two-hybrid system: an assay for protein-protein interactions.
Trends Genet. 1994 Aug;10(8):286-92 PMID: 7940758
Cooperation of proto-signals for nuclear accumulation of estrogen and progesterone receptors.
EMBO J. 1992 Oct;11(10):3681-94 PMID: 1327748
A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance.
Mol Gen Genet. 1984;197(2):345-6 PMID: 6394957
The estrogen receptor binds tightly to its responsive element as a ligand-induced homodimer.
Cell. 1988 Oct 7;55(1):145-56 PMID: 3167974
A novel genetic system to detect protein-protein interactions.
Nature. 1989 Jul 20;340(6230):245-6 PMID: 2547163
Characterization of nuclear localizing sequences derived from yeast ribosomal protein L29.
EMBO J. 1990 Jan;9(1):91-9 PMID: 2104804
Lambda YES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations.
Proc Natl Acad Sci U S A. 1991 Mar 1;88(5):1731-5 PMID: 1848010
Mxi1, a protein that specifically interacts with Max to bind Myc-Max recognition sites.
Cell. 1993 Jan 29;72(2):223-32 PMID: 8425219

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/23.5.876

Published

1995-03-11

Pages

876-8

Language

English

Region

England

NLM ID

0411011

PMCID

PMC306773

Subset

External Links

PubMed source DOI: 10.1093/nar/23.5.876 PMC Full Text View journal details

Analysis Services

A new version of the two-hybrid assay for detection of protein-protein interactions.

Contact

WeChat Official Account