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PMID: 12697820 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The yeast RSC chromatin-remodeling complex is required for kinetochore function in chromosome segregation.

Molecular and cellular biology ·Vol. 23 ·No. 9 ·2003-05-00 ·Pages 3202-15

Hsu JM, Huang J, Meluh PB, Laurent BC

Abstract

The accurate segregation of chromosomes requires the kinetochore, a complex protein machine that assembles onto centromeric DNA to mediate attachment of replicated sister chromatids to the mitotic spindle apparatus. This study reveals an important role for the yeast RSC ATP-dependent chromatin-remodeling complex at the kinetochore in chromosome transmission. Mutations in genes encoding two core subunits of RSC, the ATPase Sth1p and the Snf5p homolog Sfh1p, interact genetically with mutations in genes encoding kinetochore proteins and with a mutation in centromeric DNA. RSC also interacts genetically and physically with the histone and histone variant components of centromeric chromatin. Importantly, RSC is localized to centromeric and centromere-proximal chromosomal regions, and its association with these loci is dependent on Sth1p. Both sth1 and sfh1 mutants exhibit altered centromeric and centromere-proximal chromatin structure and increased missegregation of authentic chromosomes. Finally, RSC is not required for centromeric deposition of the histone H3 variant Cse4p, suggesting that RSC plays a role in reconfiguring centromeric and flanking nucleosomes following Cse4p recruitment for proper chromosome transmission.

MeSH Terms
Carrier Proteins/genetics,metabolism Cell Cycle Proteins/genetics,metabolism Centromere/chemistry,physiology Chromatin/genetics,metabolism,ultrastructure Chromosomal Proteins, Non-Histone Chromosome Segregation DNA-Binding Proteins/genetics,metabolism Histones/genetics Kinetochores/physiology Macromolecular Substances Membrane Proteins/genetics,metabolism Mutation Nuclear Proteins/genetics,metabolism Phospholipid Transfer Proteins Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins/genetics,metabolism Spindle Apparatus Transcription Factors/genetics,metabolism
Chemicals
CSE4 protein, S cerevisiae Carrier Proteins Cell Cycle Proteins Chromatin Chromosomal Proteins, Non-Histone DNA-Binding Proteins Histones MIF2 protein, S cerevisiae Macromolecular Substances Membrane Proteins Nuclear Proteins Phospholipid Transfer Proteins RSC complex, S cerevisiae SEC24 protein, S cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors STH1 protein, S cerevisiae
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hsu Jing-Mei
Department of Microbiology and Immunology, Morse Institute of Molecular Biology and Genetics, and Program in Molecular and Cellular Biology, State University of New York, Brooklyn, New York 11203, USA.
Huang Jian
Meluh Pamela B
Laurent Brehon C
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.23.9.3202-3215.2003
Published
2003-05-00
Pages
3202-15
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC153182
Subset
IM
Grants
NIGMS NIH HHS · R01 GM056700 · United States
NIGMS NIH HHS · GM56700 · United States
External Links
PubMed source DOI: 10.1128/MCB.23.9.3202-3215.2003 PMC Full Text View journal details
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