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PMID: 11782447 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Chromatin assembly factor I and Hir proteins contribute to building functional kinetochores in S. cerevisiae.

Genes & development ·Vol. 16 ·No. 1 ·2002-01-01 ·Pages 85-100

Sharp JA, Franco AA, Osley MA, Kaufman PD

Abstract

Budding yeast centromeres are comprised of approximately 125-bp DNA sequences that direct formation of the kinetochore, a specialized chromatin structure that mediates spindle attachment to chromosomes. We report here a novel role for the histone deposition complex chromatin assembly factor I (CAF-I) in building centromeric chromatin. The contribution of CAF-I to kinetochore function overlaps that of the Hir proteins, which have also been implicated in nucleosome formation and heterochromatic gene silencing. cacDelta hirDelta double mutant cells lacking both CAF-I and Hir proteins are delayed in anaphase entry in a spindle assembly checkpoint-dependent manner. Further, cacDelta and hirDelta deletions together cause increased rates of chromosome missegregation, genetic synergies with mutations in kinetochore protein genes, and alterations in centromeric chromatin structure. Finally, CAF-I subunits and Hir1 are enriched at centromeres, indicating that these proteins make a direct contribution to centromeric chromatin structures.

MeSH Terms
Chromatin Assembly Factor-1 Chromosomal Proteins, Non-Histone Chromosome Segregation DNA-Binding Proteins/physiology Kinetochores/physiology Mitosis/physiology Mutation Nuclear Proteins/physiology Saccharomyces cerevisiae/physiology,ultrastructure
Chemicals
Chromatin Assembly Factor-1 Chromosomal Proteins, Non-Histone DNA-Binding Proteins Nuclear Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sharp Judith A
Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA.
Franco Alexa A
Osley Mary Ann
Kaufman Paul D
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.925302
Published
2002-01-01
Pages
85-100
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC155315
Subset
IM
Grants
NIGMS NIH HHS · R01 GM040118 · United States
NIGMS NIH HHS · R01 GM055712 · United States
NIGMS NIH HHS · 1 R01 GM55712 · United States
NIGMS NIH HHS · GM40118 · United States
External Links
PubMed source DOI: 10.1101/gad.925302 PMC Full Text View journal details
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