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PMID: 8622682 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Postreplicative chromatin assembly by Drosophila and human chromatin assembly factor 1.

Molecular and cellular biology ·Vol. 16 ·No. 3 ·1996-03-00 ·Pages 810-7

Kamakaka RT, Bulger M, Kaufman PD, Stillman B, Kadonaga JT

Abstract

To study the relationship between DNA replication and chromatin assembly, we have purified a factor termed Drosophila chromatin assembly factor 1 (dCAF-1) to approximately 50% homogeneity from a nuclear extract derived from embryos. dCAF-1 appears to consist of four polypeptides with molecular masses of 180, 105, 75, and 55 kDa. dCAF-1 preferentially mediates chromatin assembly of newly replicated DNA relative to unreplicated DNA during T-antigen-dependent simian virus 40 DNA replication in vitro, as seen with human CAF-1. Analysis of the mechanism of DNA replication-coupled chromatin assembly revealed that both dCAF-1 and human CAF-1 mediate chromatin assembly preferentially with previously yet newly replicated DNA relative to unreplicated DNA. Moreover, the preferential assembly of the postreplicative DNA was observed at 30 min after inhibition of DNA replication by aphidicolin, but this effect slowly diminished until it was no longer apparent at 120 min after inhibition of replication. These findings suggest that the coupling between DNA replication and chromatin assembly may not necessarily involve a direct interaction between the replication and assembly factors at a replication fork.

MeSH Terms
Animals Chromatin/metabolism Chromatin Assembly Factor-1 Chromosomal Proteins, Non-Histone DNA Replication DNA, Viral/metabolism DNA-Binding Proteins/metabolism Drosophila/metabolism Humans Simian virus 40/genetics
Chemicals
CHAF1B protein, human Chromatin Chromatin Assembly Factor-1 Chromosomal Proteins, Non-Histone DNA, Viral DNA-Binding Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kamakaka R T
Department of Biology and Center for Molecular Genetics, University of California at San Diego, La Jolla 92093-0347, USA.
Bulger M
Kaufman P D
Stillman B
Kadonaga J T
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.16.3.810
Published
1996-03-00
Pages
810-7
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC231061
Subset
IM
Grants
NCI NIH HHS · CA13106 · United States
NIGMS NIH HHS · GM46995 · United States
External Links
PubMed source DOI: 10.1128/MCB.16.3.810 PMC Full Text View journal details
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