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PMID: 12556493 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth.

Molecular and cellular biology ·Vol. 23 ·No. 4 ·2003-02-00 ·Pages 1341-8

Vyas VK, Kuchin S, Berkey CD, Carlson M

Abstract

The Snf1 protein kinase of Saccharomyces cerevisiae has been shown to have a role in regulating haploid invasive growth in response to glucose depletion. Cells contain three forms of the Snf1 kinase, each with a different beta-subunit isoform, either Gal83, Sip1, or Sip2. We present evidence that different Snf1 kinases play distinct roles in two aspects of invasive growth, namely, adherence to the agar substrate and filamentation. The Snf1-Gal83 form of the kinase is required for adherence, whereas either Snf1-Gal83 or Snf1-Sip2 is sufficient for filamentation. Genetic evidence indicates that Snf1-Gal83 affects adherence by antagonizing Nrg1- and Nrg2-mediated repression of the FLO11 flocculin and adhesin gene. In contrast, the mechanism(s) by which Snf1-Gal83 and Snf1-Sip2 affect filamentation is independent of FLO11. Thus, the Snf1 kinase regulates invasive growth by at least two distinct mechanisms.

MeSH Terms
AMP-Activated Protein Kinases Cell Adhesion/genetics Cell Division/physiology DNA-Binding Proteins Fungal Proteins/genetics,metabolism Haploidy Isoenzymes/genetics,metabolism Membrane Glycoproteins Membrane Proteins/genetics,metabolism Mutation Protein Serine-Threonine Kinases/genetics,metabolism Protein Subunits Repressor Proteins/genetics,metabolism Saccharomyces cerevisiae/genetics,growth & development,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism Trans-Activators/genetics,metabolism
Chemicals
DNA-Binding Proteins FLO11 protein, S cerevisiae Fungal Proteins Isoenzymes Membrane Glycoproteins Membrane Proteins NRG1 protein, S cerevisiae Nrg2 protein, S cerevisiae Protein Subunits Repressor Proteins SIP2 protein, S cerevisiae Saccharomyces cerevisiae Proteins Trans-Activators SNF1-related protein kinases Protein Serine-Threonine Kinases SIP1 protein, S cerevisiae AMP-Activated Protein Kinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Vyas Valmik K
Integrated Program in Cellular, Molecular and Biophysical Studies, Columbia University, New York, New York 10032,USA.
Kuchin Sergei
Berkey Cristin D
Carlson Marian
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.23.4.1341-1348.2003
Published
2003-02-00
Pages
1341-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC141157
Subset
IM
Grants
NIGMS NIH HHS · R01 GM034095 · United States
NIGMS NIH HHS · R37 GM034095 · United States
NIGMS NIH HHS · GM34095 · United States
External Links
PubMed source DOI: 10.1128/MCB.23.4.1341-1348.2003 PMC Full Text View journal details
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