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PMID: 11532939 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

NRG1, a repressor of filamentous growth in C.albicans, is down-regulated during filament induction.

The EMBO journal ·Vol. 20 ·No. 17 ·2001-09-03 ·Pages 4753-61

Braun BR, Kadosh D, Johnson AD

Abstract

In response to a variety of external signals, the fungal pathogen Candida albicans undergoes a transition between ellipsoidal single cells (blastospores) and filaments composed of elongated cells attached end-to-end. Here we identify a DNA-binding protein, Nrg1, that represses filamentous growth in Candida probably by acting through the co-repressor Tup1. nrg1 mutant cells are predominantly filamentous under non-filament-inducing conditions and their colony morphology resembles that of tup1 mutants. We also identify two filament-specific genes, ECE1 and HWP1, whose transcription is repressed by Nrg1 under non-inducing conditions. These genes constitute a subset of those under Tup1 control, providing further evidence that Nrg1 acts by recruiting Tup1 to target genes. We show that growth in serum at 37 degrees C, a potent inducer of filamentous growth, causes a reduction of NRG1 mRNA, suggesting that filamentous growth is induced by the down-regulation of NRG1. Consistent with this idea, expression of NRG1 from a non-regulated promoter partially blocks the induction of filamentous growth.

MeSH Terms
Amino Acid Sequence Binding Sites Candida/genetics,growth & development,pathogenicity Culture Media DNA-Binding Proteins Fungal Proteins/genetics,metabolism Gene Deletion Gene Expression Regulation, Fungal Membrane Glycoproteins/genetics Molecular Sequence Data Promoter Regions, Genetic Repressor Proteins/chemistry,genetics,metabolism Saccharomyces cerevisiae/genetics,growth & development Saccharomyces cerevisiae Proteins Sequence Alignment Sequence Homology, Amino Acid Transcription, Genetic Virulence Zinc Fingers
Chemicals
Culture Media DNA-Binding Proteins ECE1 protein, Candida albicans Fungal Proteins HWP1 protein, Candida albicans Membrane Glycoproteins NRG1 protein, S cerevisiae Repressor Proteins Saccharomyces cerevisiae Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Braun B R
Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, CA 94143-0414, USA.
Kadosh D
Johnson A D
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/20.17.4753
Published
2001-09-03
Pages
4753-61
Language
English
Region
England
NLM ID
8208664
PMCID
PMC125265
Subset
IM
Grants
NIGMS NIH HHS · R01 GM037049 · United States
NIGMS NIH HHS · GM37049 · United States
External Links
PubMed source DOI: 10.1093/emboj/20.17.4753 PMC Full Text View journal details
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