Home LiteratureArticle Details
PMID: 9832517 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Characterization of three related glucose repressors and genes they regulate in Saccharomyces cerevisiae.

Genetics ·Vol. 150 ·No. 4 ·1998-12-00 ·Pages 1377-91

Lutfiyya LL, Iyer VR, DeRisi J, DeVit MJ, Brown PO, Johnston M

Abstract

Mig1 and Mig2 are proteins with similar zinc fingers that are required for glucose repression of SUC2 expression. Mig1, but not Mig2, is required for repression of some other glucose-repressed genes, including the GAL genes. A second homolog of Mig1, Yer028, appears to be a glucose-dependent transcriptional repressor that binds to the Mig1-binding sites in the SUC2 promoter, but is not involved in glucose repression of SUC2 expression. Despite their functional redundancy, we found several significant differences between Mig1 and Mig2: (1) in the absence of glucose, Mig1, but not Mig2, is inactivated by the Snf1 protein kinase; (2) nuclear localization of Mig1, but not Mig2, is regulated by glucose; (3) expression of MIG1, but not MIG2, is repressed by glucose; and (4) Mig1 and Mig2 bind to similar sites but with different relative affinities. By two approaches, we have identified many genes regulated by Mig1 and Mig2, and confirmed a role for Mig1 and Mig2 in repression of several of them. We found no genes repressed by Yer028. Also, we identified no genes repressed by only Mig1 or Mig2. Thus, Mig1 and Mig2 are redundant glucose repressors of many genes.

MeSH Terms
Amino Acid Sequence Binding Sites DNA-Binding Proteins/genetics,metabolism,physiology Gene Expression Regulation, Fungal Genes, Fungal Glucose Glycoside Hydrolases/genetics Molecular Sequence Data Promoter Regions, Genetic Repressor Proteins/genetics,metabolism,physiology Saccharomyces cerevisiae/genetics,metabolism,physiology Saccharomyces cerevisiae Proteins Transcription, Genetic Zinc Fingers beta-Fructofuranosidase
Chemicals
DNA-Binding Proteins MIG1 protein, S cerevisiae Repressor Proteins Saccharomyces cerevisiae Proteins Glycoside Hydrolases beta-Fructofuranosidase Glucose
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Lutfiyya L L
Department of Genetics, Washington University School of Medicine, St. Louis, Missouri 63110, USA. mj@genetics.wustl.edu
Iyer V R
DeRisi J
DeVit M J
Brown P O
Johnston M
References (42)
42 references, click to expand
  1. RNA from the yeast transposable element Ty1 has both ends in the direct repeats, a structure similar to retrovirus RNA.
    Proc Natl Acad Sci U S A. 1983 May;80(9):2432-6 PMID: 6189122
  2. Glucose control in Saccharomyces cerevisiae: the role of Mig1 in metabolic functions.
    Microbiology. 1998 Jan;144 ( Pt 1):13-24 PMID: 9467897
  3. Use of lacZ fusions to delimit regulatory elements of the inducible divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae.
    Mol Cell Biol. 1984 Oct;4(10):1985-98 PMID: 6390181
  4. Saccharomyces cerevisiae GAL1-GAL10 divergent promoter region: location and function of the upstream activating sequence UASG.
    Mol Cell Biol. 1984 Nov;4(11):2467-78 PMID: 6392852
  5. Upstream activation sites of the CYC1 gene of Saccharomyces cerevisiae are active when inverted but not when placed downstream of the "TATA box".
    Proc Natl Acad Sci U S A. 1984 Dec;81(24):7860-4 PMID: 6096863
  6. Upstream region required for regulated expression of the glucose-repressible SUC2 gene of Saccharomyces cerevisiae.
    Mol Cell Biol. 1984 Dec;4(12):2750-7 PMID: 6396505
  7. A yeast gene that is essential for release from glucose repression encodes a protein kinase.
    Science. 1986 Sep 12;233(4769):1175-80 PMID: 3526554
  8. Promoters selected from random DNA sequences.
    Proc Natl Acad Sci U S A. 1986 Oct;83(19):7405-9 PMID: 3532112
  9. Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions.
    Gene. 1986;45(3):299-310 PMID: 3026915
  10. DNA specificity of the bicoid activator protein is determined by homeodomain recognition helix residue 9.
    Cell. 1989 Jun 30;57(7):1275-83 PMID: 2500253
  11. A single amino acid can determine the DNA binding specificity of homeodomain proteins.
    Cell. 1989 Nov 3;59(3):553-62 PMID: 2572327
  12. High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier.
    Curr Genet. 1989 Dec;16(5-6):339-46 PMID: 2692852
  13. Yeast MIG1 repressor is related to the mammalian early growth response and Wilms' tumour finger proteins.
    EMBO J. 1990 Sep;9(9):2891-8 PMID: 2167835
  14. Zinc finger-DNA recognition: crystal structure of a Zif268-DNA complex at 2.1 A.
    Science. 1991 May 10;252(5007):809-17 PMID: 2028256
  15. Regulated expression of the GAL4 activator gene in yeast provides a sensitive genetic switch for glucose repression.
    Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8597-601 PMID: 1924319
  16. Regions in the promoter of the yeast FBP1 gene implicated in catabolite repression may bind the product of the regulatory gene MIG1.
    FEBS Lett. 1991 Oct 7;291(1):97-100 PMID: 1657641
  17. Glucose repression in the yeast Saccharomyces cerevisiae.
    Mol Microbiol. 1992 Jan;6(1):15-21 PMID: 1310793
  18. Alteration by phenobarbital and 3-methyl-cholanthrene of functional and structural changes in rat liver due to carbon tetrachloride inhalation.
    J Pharmacol Exp Ther. 1975 Apr;193(1):281-92 PMID: 1133769
  19. Beta-D-fructofuranoside fructohydrolase from yeast.
    Methods Enzymol. 1975;42:504-11 PMID: 237205
  20. Ssn6-Tup1 is a general repressor of transcription in yeast.
    Cell. 1992 Feb 21;68(4):709-19 PMID: 1739976
  21. Analysis of URSG-mediated glucose repression of the GAL1 promoter of Saccharomyces cerevisiae.
    Genetics. 1992 Feb;130(2):295-304 PMID: 1541392
  22. Zinc finger-DNA recognition: analysis of base specificity by site-directed mutagenesis.
    Nucleic Acids Res. 1992 Aug 25;20(16):4137-44 PMID: 1508708
  23. CASTing for multicomponent DNA-binding complexes.
    Trends Biochem Sci. 1993 Mar;18(3):77-80 PMID: 8386867
  24. A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae.
    Nucleic Acids Res. 1993 Jul 11;21(14):3329-30 PMID: 8341614
  25. Importance of a flanking AT-rich region in target site recognition by the GC box-binding zinc finger protein MIG1.
    Mol Cell Biol. 1994 Mar;14(3):1979-85 PMID: 8114729
  26. Homeodomain-DNA recognition.
    Cell. 1994 Jul 29;78(2):211-23 PMID: 8044836
  27. Synergistic release from glucose repression by mig1 and ssn mutations in Saccharomyces cerevisiae.
    Genetics. 1994 May;137(1):49-54 PMID: 8056322
  28. Three different regulatory mechanisms enable yeast hexose transporter (HXT) genes to be induced by different levels of glucose.
    Mol Cell Biol. 1995 Mar;15(3):1564-72 PMID: 7862149
  29. CAT8, a new zinc cluster-encoding gene necessary for derepression of gluconeogenic enzymes in the yeast Saccharomyces cerevisiae.
    Mol Cell Biol. 1995 Apr;15(4):1915-22 PMID: 7891685
  30. DNA-binding specificity of NGFI-A and related zinc finger transcription factors.
    Mol Cell Biol. 1995 Apr;15(4):2275-87 PMID: 7891721
  31. Glucose repression in fungi.
    Trends Genet. 1995 Jan;11(1):12-7 PMID: 7900189
  32. MIG1-dependent and MIG1-independent glucose regulation of MAL gene expression in Saccharomyces cerevisiae.
    Curr Genet. 1995 Aug;28(3):258-66 PMID: 8529272
  33. Determinants of DNA-binding specificity of ETS-domain transcription factors.
    Mol Cell Biol. 1996 Jul;16(7):3338-49 PMID: 8668149
  34. Two zinc-finger-containing repressors are responsible for glucose repression of SUC2 expression.
    Mol Cell Biol. 1996 Sep;16(9):4790-7 PMID: 8756637
  35. Removal of Mig1p binding site converts a MAL63 constitutive mutant derived by interchromosomal gene conversion to glucose insensitivity.
    Genetics. 1996 Jan;142(1):51-63 PMID: 8770584
  36. Green fluorescent protein as a marker for gene expression and subcellular localization in budding yeast.
    Yeast. 1996 Jun 30;12(8):773-86 PMID: 8813764
  37. Alleviation of glucose repression of maltose metabolism by MIG1 disruption in Saccharomyces cerevisiae.
    Appl Environ Microbiol. 1996 Dec;62(12):4441-9 PMID: 8953715
  38. Variations of the C2H2 zinc finger motif in the yeast genome and classification of yeast zinc finger proteins.
    Nucleic Acids Res. 1997 Jun 15;25(12):2464-9 PMID: 9171100
  39. Molecular evidence for an ancient duplication of the entire yeast genome.
    Nature. 1997 Jun 12;387(6634):708-13 PMID: 9192896
  40. Exploring the metabolic and genetic control of gene expression on a genomic scale.
    Science. 1997 Oct 24;278(5338):680-6 PMID: 9381177
  41. Identification of cis-acting elements in the SUC2 promoter of Saccharomyces cerevisiae required for activation of transcription.
    Nucleic Acids Res. 1998 Feb 15;26(4):1002-9 PMID: 9461460
  42. Cloning and genetic mapping of SNF1, a gene required for expression of glucose-repressible genes in Saccharomyces cerevisiae.
    Mol Cell Biol. 1984 Jan;4(1):49-53 PMID: 6366512
Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/150.4.1377
Email
mj@genetics.wustl.edu
Published
1998-12-00
Pages
1377-91
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1460414
Subset
IM
Grants
NIGMS NIH HHS · R01 GM032540 · United States
NIGMS NIH HHS · GM32540 · United States
External Links
PubMed source DOI: 10.1093/genetics/150.4.1377 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2