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PMID: 10022891 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nrg1 is a transcriptional repressor for glucose repression of STA1 gene expression in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 19 ·No. 3 ·1999-03-00 ·Pages 2044-50

Park SH, Koh SS, Chun JH, Hwang HJ, Kang HS

Abstract

Expression of genes encoding starch-degrading enzymes is regulated by glucose repression in the yeast Saccharomyces cerevisiae. We have identified a transcriptional repressor, Nrg1, in a genetic screen designed to reveal negative factors involved in the expression of STA1, which encodes a glucoamylase. The NRG1 gene encodes a 25-kDa C2H2 zinc finger protein which specifically binds to two regions in the upstream activation sequence of the STA1 gene, as judged by gel retardation and DNase I footprinting analyses. Disruption of the NRG1 gene causes a fivefold increase in the level of the STA1 transcript in the presence of glucose. The expression of NRG1 itself is inhibited in the absence of glucose. DNA-bound LexA-Nrg1 represses transcription of a target gene 10.7-fold in a glucose-dependent manner, and this repression is abolished in both ssn6 and tup1 mutants. Two-hybrid and glutathione S-transferase pull-down experiments show an interaction of Nrg1 with Ssn6 both in vivo and in vitro. These findings indicate that Nrg1 acts as a DNA-binding repressor and mediates glucose repression of the STA1 gene expression by recruiting the Ssn6-Tup1 complex.

MeSH Terms
Amino Acid Sequence Base Sequence Culture Media DNA, Fungal DNA-Binding Proteins/genetics Fungal Proteins/genetics,metabolism Gene Expression Regulation, Fungal/drug effects Glucose/pharmacology Molecular Sequence Data Promoter Regions, Genetic Repressor Proteins/genetics,metabolism STAT1 Transcription Factor Saccharomyces cerevisiae/drug effects,genetics,metabolism Saccharomyces cerevisiae Proteins Trans-Activators/genetics Zinc Fingers
Chemicals
Culture Media DNA, Fungal DNA-Binding Proteins Fungal Proteins NRG1 protein, S cerevisiae Repressor Proteins STAT1 Transcription Factor Saccharomyces cerevisiae Proteins Trans-Activators Glucose
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Park S H
Department of Microbiology, College of Natural Sciences, Seoul National University, Seoul 151-742, Korea.
Koh S S
Chun J H
Hwang H J
Kang H S
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.19.3.2044
Published
1999-03-00
Pages
2044-50
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC83997
Subset
IM
Databases
GENBANK
Z49812
External Links
PubMed source DOI: 10.1128/MCB.19.3.2044 PMC Full Text View journal details
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