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PMID: 9811878 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The three yeast A kinases have specific signaling functions in pseudohyphal growth.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 95 ·No. 23 ·1998-11-10 ·Pages 13783-7

Robertson LS, Fink GR

Abstract

The three yeast A kinase catalytic subunit isoforms are redundant for viability. We demonstrate that they have dramatically different roles in pseudohyphal development: Tpk2 is essential, whereas Tpk3 inhibits. Tpk1 has no discernible effect. Two-hybrid analysis identified the transcription factor Sfl1 as a protein that interacts specifically with Tpk2, but not Tpk1 or Tpk3. Deletion of SFL1 enhances pseudohyphal and invasive growth. Flo11, a cell surface flocculin required for pseudohyphal development, is transcriptionally regulated by Tpk2 and Sfl1. Genetic evidence indicates that Tpk2 acts upstream of Sfl1 in the regulation of Flo11.

MeSH Terms
Cyclic AMP-Dependent Protein Kinases/physiology Gene Expression Regulation, Enzymologic Gene Expression Regulation, Fungal Mutation Saccharomyces cerevisiae/enzymology,genetics,growth & development Signal Transduction
Chemicals
Cyclic AMP-Dependent Protein Kinases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Robertson L S
Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA.
Fink G R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.95.23.13783
Published
1998-11-10
Pages
13783-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC24897
Subset
IM
Grants
NHGRI NIH HHS · T32 HG000039 · United States
NHGRI NIH HHS · HG0039 · United States
NIGMS NIH HHS · R01 GM040266 · United States
NIGMS NIH HHS · GM35101 · United States
NIGMS NIH HHS · GM40266 · United States
External Links
PubMed source DOI: 10.1073/pnas.95.23.13783 PMC Full Text View journal details
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