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PMID: 12151506 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cortical excitatory neurons and glia, but not GABAergic neurons, are produced in the Emx1-expressing lineage.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 22 ·No. 15 ·2002-08-01 ·Pages 6309-14

Gorski JA, Talley T, Qiu M, Puelles L, Rubenstein JL, Jones KR

Abstract

By homologous recombination of an internal ribosome entry site and Cre recombinase coding region into the 3'-untranslated region of the mouse Emx1 gene, we have generated a strain of mice, Emx1(IRES)cre, that expresses the Cre recombinase in a spatial and temporal pattern like that observed for Emx1. When mated to reporter strains, these mice are a sensitive means to fate-map the Emx1-expressing cells of the developing forebrain. Our results demonstrate that radial glia, Cajal-Retzius cells, glutamatergic neurons, astrocytes, and oligodendrocytes of most pallial structures originate from an Emx1-expressing lineage. On the other hand, most of the pallial GABAergic neurons arise outside the Emx1-expressing lineage. Structures that are located near the basal ganglia (e.g., the amygdala and endopiriform nuclei) are not uniformly derived from Emx1-expressing cells.

MeSH Terms

3' Untranslated Regions/genetics Amygdala/cytology,embryology Animals Basal Ganglia/cytology,embryology Cell Lineage Cerebral Cortex/cytology,embryology Gene Targeting Genes, Reporter Homeodomain Proteins/biosynthesis,genetics Integrases/biosynthesis,genetics Interneurons/cytology Mice Mice, Mutant Strains Models, Animal Neuroglia/cytology Neurons/cytology,metabolism Recombination, Genetic Stem Cells/cytology,metabolism Telencephalon/cytology,embryology Transcription Factors Viral Proteins/biosynthesis,genetics gamma-Aminobutyric Acid/metabolism

Chemicals

3' Untranslated Regions Homeodomain Proteins Transcription Factors Viral Proteins empty spiracles homeobox proteins gamma-Aminobutyric Acid Cre recombinase Integrases

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Gorski Jessica A

Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309, USA.

Talley Tiffany

Qiu Mengsheng

Puelles Luis

Rubenstein John L R

Jones Kevin R

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Article Info

Journal

The Journal of neuroscience : the official journal of the Society for Neuroscience

Abbr.

J Neurosci

ISSN

1529-2401

DOI

20026564

Published

2002-08-01

Pages

6309-14

Language

English

Region

United States

NLM ID

8102140

PMCID

PMC6758181

Subset

Grants

Wellcome Trust · United Kingdom

External Links

PubMed source DOI: 20026564 PMC Full Text View journal details

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Cortical excitatory neurons and glia, but not GABAergic neurons, are produced in the Emx1-expressing lineage.

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