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PMID: 12127789 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Functional properties of the predicted helicase of porcine reproductive and respiratory syndrome virus.

Virology ·Vol. 298 ·No. 2 ·2002-07-05 ·Pages 258-70

Bautista EM, Faaberg KS, Mickelson D, McGruder ED

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) is a member of the positive-strand RNA virus family Arteriviridae. Although considerable research has focused on this important pathogen, little is known about the function of most PRRSV proteins. To examine characteristics of putative nonstructural proteins (nsp) encoded in ORF1b, which have been identified by nucleotide similarity to domains of equine arteritis virus, defined genomic regions were cloned and expressed in the pRSET expression system. One region, nsp10, encoded a protein with a putative helicase domain and was further examined for functional helicase-like activities. PRRSV nsp10 was found to possess a thermolabile and pH-sensitive NTPase activity that was modulated by polynucleotides and to unwind dsRNA in a 5' to 3' polarity. These results provide the first evidence of the functional properties of PRRSV helicase and further support the finding that nidovirus helicases possess properties that distinguish them from other viral helicases.

MeSH Terms
3' Untranslated Regions 5' Untranslated Regions Acid Anhydride Hydrolases/metabolism Adenosine Triphosphatases/metabolism Gene Expression Hydrogen-Ion Concentration Nucleoside-Triphosphatase Open Reading Frames Porcine respiratory and reproductive syndrome virus/enzymology RNA Helicases/metabolism RNA, Viral/genetics,metabolism Recombinant Proteins/biosynthesis,metabolism Temperature Viral Nonstructural Proteins/genetics,metabolism Virus Replication
Chemicals
3' Untranslated Regions 5' Untranslated Regions RNA, Viral Recombinant Proteins Viral Nonstructural Proteins Acid Anhydride Hydrolases Adenosine Triphosphatases Nucleoside-Triphosphatase RNA Helicases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bautista Elida M
Elanco Animal Health Research and Development, a Division of Eli Lilly and Company, Greenfield, IN 64140, USA. Ebautista@piadc.ars.usda.gov
Faaberg Kay S
Mickelson Dan
McGruder Edward D
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Article Info
Journal
Virology
Abbr.
Virology
ISSN
0042-6822
DOI
10.1006/viro.2002.1495
Email
ebautista@piadc.ars.usda.gov
Published
2002-07-05
Pages
258-70
Language
English
Region
United States
NLM ID
0110674
PMCID
PMC7130902
Subset
IM
External Links
PubMed source DOI: 10.1006/viro.2002.1495 PMC Full Text View journal details
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