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PMID: 8892948 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

ATPase, GTPase, and RNA binding activities associated with the 206-kilodalton protein of turnip yellow mosaic virus.

Journal of virology ·Vol. 70 ·No. 11 ·1996-11-00 ·Pages 8169-74

Kadaré G, David C, Haenni AL

Abstract

The 206-kDa protein of turnip yellow mosaic virus belongs to an expanding group of proteins containing a domain which includes the consensus nucleotide binding site GxxxxGKS/T. A portion of this protein (amino acids [aa] 916 to 1259) was expressed in Escherichia coli and purified by affinity chromatography to near homogeneity. In the absence of any other viral factors, it exhibited ATPase and GTPase activities in vitro. A mutant protein with a single amino acid substitution in the consensus nucleotide binding site (Lys-982 to Ser) exhibited only low levels of both activities, implying that Lys-982 is important for nucleoside triphosphatase activity. The protein also possessed nonspecific RNA binding capacity. Deletion mutants revealed that an N-terminal domain (aa 916 to 1061) and a C-terminal domain (aa 1182 to 1259) participate in RNA binding. The results presented here provide the first experimental evidence that turnip yellow mosaic virus encodes nucleoside triphosphatase and RNA binding activities.

MeSH Terms
Adenosine Triphosphatases/genetics,isolation & purification,metabolism Cloning, Molecular Conserved Sequence GTP Phosphohydrolases/genetics,isolation & purification,metabolism Gene Expression Lysine Mutagenesis, Site-Directed RNA-Binding Proteins/genetics,isolation & purification,metabolism Recombinant Fusion Proteins/genetics,isolation & purification,metabolism Serine Tymovirus/genetics,metabolism Viral Proteins/genetics,isolation & purification,metabolism
Chemicals
RNA-Binding Proteins Recombinant Fusion Proteins Viral Proteins Serine Adenosine Triphosphatases GTP Phosphohydrolases Lysine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kadaré G
Institut Jacques Monod, Paris, France.
David C
Haenni A L
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41 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.70.11.8169-8174.1996
Published
1996-11-00
Pages
8169-74
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC190897
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.70.11.8169-8174.1996 PMC Full Text View journal details
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