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PMID: 11894917 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Post-transcriptional control of expression of the repA gene of plasmid R1 mediated by a small RNA molecule.

The EMBO journal ·Vol. 2 ·No. 1 ·1983-00-00 ·Pages 93-8

Light J, Molin S

Abstract

Fusions between the repA gene of plasmid R1 (required for autonomous plasmid replication) and the lac genes have been the basis for in vivo studies of regulation of repA expression. Two gene products--the CopA RNA and the CopB protein--act as inhibitors of repA expression. Comparison of the effects of addition in trans of the two Cop functions on transcription and translation of repA-lac gene fusions show that the CopB protein represses transcription of the repA gene, whereas the CopA RNA interferes with the RepA mRNA in such a way that effective translation is inhibited. The CopA RNA does not seem to have a direct effect on the transcription of the repA gene but, as a consequence of the posttranscriptional regulation, transcriptional polarity within the repA gene is observed. It is also shown that the CopA RNA interacts with its target (CopT) only when the region is transcribed to form RepA mRNA.

MeSH Terms
Artificial Gene Fusion Bacterial Proteins/genetics DNA, Bacterial Escherichia coli Proteins Gene Expression Gene Expression Regulation, Bacterial Genes, Bacterial Lac Operon Membrane Transport Proteins/genetics Monosaccharide Transport Proteins Plasmids Protein Biosynthesis RNA Processing, Post-Transcriptional RNA, Bacterial/metabolism RNA, Messenger Symporters Transcription Factors/genetics Transcription, Genetic
Chemicals
Bacterial Proteins CopA protein, Bacteria DNA, Bacterial Escherichia coli Proteins LacY protein, E coli Membrane Transport Proteins Monosaccharide Transport Proteins RNA, Bacterial RNA, Messenger Symporters Transcription Factors lactose permease lemA protein, bacterial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Light J
Department of Molecular Biology, Odense University, Denmark.
Molin S
References (24)
24 references, click to expand
  1. Vertical dye-buoyant density gradients for rapid analysis and preparation of plasmid DNA.
    Anal Biochem. 1981 Nov 15;118(1):191-3 PMID: 7337211
  2. Replication control functions of plasmid R1 act as inhibitors of expression of a gene required for replication.
    Mol Gen Genet. 1981;184(1):56-61 PMID: 7038389
  3. Clustering of genes involved in replication, copy number control, incompatibility, and stable maintenance of the resistance plasmid R1drd-19.
    J Bacteriol. 1979 Apr;138(1):70-9 PMID: 374378
  4. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli.
    J Mol Biol. 1980 Apr;138(2):179-207 PMID: 6997493
  5. The nucleotide sequence of the replication control region of the resistance plasmid R1drd-19.
    Mol Gen Genet. 1981;181(1):116-22 PMID: 6261081
  6. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.
    Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4 PMID: 4559594
  7. Regulation of DNA replication: "target" determinant of the replication control elements of plasmid R6-5 lies within a control element gene.
    Proc Natl Acad Sci U S A. 1981 Aug;78(8):4699-703 PMID: 7029525
  8. Plasmid vehicles for direct cloning of Escherichia coli promoters.
    J Bacteriol. 1979 Nov;140(2):400-7 PMID: 387734
  9. Isolation and characterization of new copy mutants of plasmid R1, and identification of a polypeptide involved in copy number control.
    Mol Gen Genet. 1981;181(1):123-30 PMID: 6261082
  10. Analysis of plasmid genome evolution based on nucleotide-sequence comparison of two related plasmids of Escherichia coli.
    Gene. 1982 Mar;17(3):299-310 PMID: 6286411
  11. Isolation and characterization of the minimal fragment required for autonomous replication ("basic replicon") of a copy mutant (pKN102) of the antibiotic resistance factor R1.
    Mol Gen Genet. 1978 Jun 1;162(1):51-7 PMID: 353525
  12. Molecular cloning and functional characterization of a copy number control gene (copB) of plasmid R1.
    J Bacteriol. 1982 Sep;151(3):1136-45 PMID: 7050078
  13. Maximizing gene expression on a plasmid using recombination in vitro.
    Cell. 1978 Jan;13(1):65-71 PMID: 340049
  14. In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals.
    J Bacteriol. 1980 Aug;143(2):971-80 PMID: 6162838
  15. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  16. Isolation and characterization of ColE1-derived plasmid copy-number mutant.
    Proc Natl Acad Sci U S A. 1978 Dec;75(12):5869-73 PMID: 104293
  17. Plasmid ColE1 incompatibility determined by interaction of RNA I with primer transcript.
    Proc Natl Acad Sci U S A. 1981 Oct;78(10):6096-100 PMID: 6171811
  18. The structure of tandem regulatory regions in the deo operon of Escherichia coli K12.
    EMBO J. 1982;1(3):317-22 PMID: 16453417
  19. RNAs involved in copy-number control and incompatibility of plasmid R1.
    Proc Natl Acad Sci U S A. 1981 Oct;78(10):6008-12 PMID: 6171808
  20. The sites of action of the two copy number control functions of plasmid R1.
    Mol Gen Genet. 1982;187(3):486-93 PMID: 6294474
  21. Plasmid R1 DNA replication dependent on protein synthesis in cell-free extracts of E. coli.
    Nature. 1981 Jan 22;289(5795):326-8 PMID: 7005697
  22. A runaway-replication mutant of plasmid R1drd-19: temperature-dependent loss of copy number control.
    Mol Gen Genet. 1978 Oct 4;165(2):167-79 PMID: 366376
  23. Expression of ribosomal genes in bacteria.
    Adv Genet. 1982;21:53-121 PMID: 7036693
  24. Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli.
    J Bacteriol. 1951 Sep;62(3):293-300 PMID: 14888646
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1983-00-00
Pages
93-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC555093
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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