Home LiteratureArticle Details
PMID: 7029525 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulation of DNA replication: "target" determinant of the replication control elements of plasmid R6-5 lies within a control element gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 8 ·1981-08-00 ·Pages 4699-703

Danbara H, Brady G, Timmis JK, Timmis KN

Abstract

The replication control system of plasmid R6-5 has been investigated by characterization of high-copy-number mutant miniplasmids, development of an in vivo assay for the site of action or "target" of the replication control elements, and sequence analysis of the replication control regions of the wild-type plasmid and two copy-number mutant derivatives. These and other experiments have shown that three plasmid determinants--copA/incA, copB, and copT--are involved in DNA replication control. The products of the copB and copA/incA genes, a 9500-dalton basic polypeptide and either a 7200-dalton basic polypeptide or a short untranslated RNA molecule, respectively, are negative-acting elements that interact with the third element, their target, the copT DNA sequence, or its product to regulate the frequency of initiation of plasmid replication. The location of copT within the copA/incA gene and 1600 base pairs upstream from the origin of replication indicates that regulation is effected at a preinitiation stage of replication, such as the production of a primer or other initiation factor.

MeSH Terms
Base Sequence Chromosome Mapping DNA Replication Escherichia coli/genetics Genes, Regulator Mutation Nucleic Acid Conformation Plasmids Replicon
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Danbara H
Brady G
Timmis J K
Timmis K N
References (33)
33 references, click to expand
  1. Replication control in a composite plasmid constructed by in vitro linkage of two distinct replicons.
    Nature. 1976 Jan 29;259(5541):285-90 PMID: 765836
  2. Plasmid incompatibility and control of replication: copy mutants of the R-factor R1 in Escherichia coli K-12.
    J Bacteriol. 1975 Nov;124(2):641-9 PMID: 1102525
  3. Isolation, by tetracycline selection, of small plasmids derived from R-factor R12 in Escherichia coli K-12.
    J Bacteriol. 1976 Jul;127(1):644-55 PMID: 776944
  4. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  5. Origin and direction of replication of the drug resistance plasmid R100.1 and of a resistance transfer factor derivative in synchronized cultures.
    J Bacteriol. 1977 Sep;131(3):929-42 PMID: 330504
  6. Amino acid sequence of Cro regulatory protein of bacteriophage lambda.
    Nature. 1977 Nov 17;270(5634):275-7 PMID: 593348
  7. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  8. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
    J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
  9. Isolation and characterization of the minimal fragment required for autonomous replication ("basic replicon") of a copy mutant (pKN102) of the antibiotic resistance factor R1.
    Mol Gen Genet. 1978 Jun 1;162(1):51-7 PMID: 353525
  10. Cloning and characterization of EcoRI and HindIII restriction endonuclease-generated fragments of antibiotic resistance plasmids R6-5 and R6.
    Mol Gen Genet. 1978 Jun 14;162(2):121-37 PMID: 672900
  11. Plasmid incompatibility: cloning analysis of an incFII determinant of R6-5.
    Nature. 1978 May 4;273(5657):27-32 PMID: 692666
  12. Structural and functional analysis of cloned DNA segments containing the replication and incompatibility regions of a miniplasmid derived from a copy number mutant of NR1.
    J Bacteriol. 1979 Jan;137(1):92-104 PMID: 368043
  13. Plasmid replication functions. II. Cloning analysis of the repA replication region of antibiotic resistance plasmid R6-5.
    Mol Gen Genet. 1979 Jan 5;168(1):1-25 PMID: 372736
  14. Plasmid replication functions. III. Origin and direction of replication of a "mini" plasmid derived from R6-5.
    Mol Gen Genet. 1979 Jan 5;168(1):27-36 PMID: 372738
  15. A rapid method for the identification of plasmid desoxyribonucleic acid in bacteria.
    Plasmid. 1978 Sep;1(4):584-8 PMID: 107540
  16. Unidirectional replication in Escherichia coli of three small plasmids derived from R factor R12.
    Plasmid. 1977 Nov;1(1):8-18 PMID: 375272
  17. Genetic and physical studies on the replication of ColE1-type plasmids.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:69-76 PMID: 383386
  18. Control of plasmid R1 replication: functions involved in replication, copy number control, incompatibility, and switch-off of replication.
    J Bacteriol. 1980 Jan;141(1):111-20 PMID: 6986353
  19. Cloning of replication, incompatibility, and stability functions of R plasmid NR1.
    J Bacteriol. 1980 Jan;141(1):87-99 PMID: 6243631
  20. Regulatory sequences involved in the promotion and termination of RNA transcription.
    Annu Rev Genet. 1979;13:319-53 PMID: 94251
  21. Formation of an RNA primer for initiation of replication of ColE1 DNA by ribonuclease H.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2450-4 PMID: 6156450
  22. Plasmid replication functions: two distinct segments of plasmid R1, RepA and RepD, express incompatibility and are capable of autonomous replication.
    J Bacteriol. 1980 Dec;144(3):1126-38 PMID: 6254944
  23. Genes and sites involved in replication and incompatibility of an R100 plasmid derivative based on nucleotide sequence analysis.
    Mol Gen Genet. 1980;179(3):527-37 PMID: 7003300
  24. Runaway replication of plasmid R1 is not caused by loss of replication inhibitor activity of gene cop.
    J Bacteriol. 1980 Aug;143(2):1046-8 PMID: 7009544
  25. Incompatibility properties of Col E1 and pMB1 derivative plasmids: random replication of multicopy replicons.
    Cell. 1981 Jan;23(1):229-38 PMID: 7011568
  26. Role of RNA transcripts in replication incompatibility and copy number control in antibiotic resistance plasmid derivatives.
    Nature. 1981 Apr 30;290(5809):794-7 PMID: 6163994
  27. Inheritance functions of group IncFII transmissible antibiotic resistance plasmids.
    Plasmid. 1981 Jan;5(1):53-75 PMID: 7012873
  28. Mutations in R factors of Escherichia coli causing an increased number of R-factor copies per chromosome.
    J Bacteriol. 1972 May;110(2):562-9 PMID: 4553836
  29. Nonchromosomal antibiotic resistance in bacteria. V. Isolation and characterization of R factor mutants exhibiting temperature-sensitive repression of fertility.
    J Bacteriol. 1972 Jun;110(3):1082-8 PMID: 4555403
  30. Identification and mapping of the replication genes of an R factor, R100-1, integrated into the chromosome of Escherichia coli K-12.
    J Bacteriol. 1974 Jun;118(3):1123-31 PMID: 4597997
  31. Round of replication mutant of a drug resistance factor.
    J Bacteriol. 1974 Jun;118(3):855-66 PMID: 4598007
  32. Class of small multicopy plasmids originating from the mutant antibiotic resistance factor R1 drd-19B2.
    J Bacteriol. 1975 Aug;123(2):658-65 PMID: 1097421
  33. Genetic structure and regulation of a replicon of plasmid lambdadv.
    J Mol Biol. 1976 Apr 15;102(3):427-39 PMID: 1271469
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.8.4699
Published
1981-08-00
Pages
4699-703
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC320230
Subset
IM
Databases
GENBANK
J01775
External Links
PubMed source DOI: 10.1073/pnas.78.8.4699 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2