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PMID: 11779812 Published · ppublish English Journal Article

Insertional mutagenesis of genes required for seed development in Arabidopsis thaliana.

Genetics ·Vol. 159 ·No. 4 ·2001-12-00 ·Pages 1751-63

McElver J, Tzafrir I, Aux G, Rogers R, Ashby C, Smith K, Thomas C, Schetter A, Zhou Q, Cushman MA, Tossberg J, Nickle T, Levin JZ, Law M, Meinke D, Patton D

Abstract

The purpose of this project was to identify large numbers of Arabidopsis genes with essential functions during seed development. More than 120,000 T-DNA insertion lines were generated following Agrobacterium-mediated transformation. Transgenic plants were screened for defective seeds and putative mutants were subjected to detailed analysis in subsequent generations. Plasmid rescue and TAIL-PCR were used to recover plant sequences flanking insertion sites in tagged mutants. More than 4200 mutants with a wide range of seed phenotypes were identified. Over 1700 of these mutants were analyzed in detail. The 350 tagged embryo-defective (emb) mutants identified to date represent a significant advance toward saturation mutagenesis of EMB genes in Arabidopsis. Plant sequences adjacent to T-DNA borders in mutants with confirmed insertion sites were used to map genome locations and establish tentative identities for 167 EMB genes with diverse biological functions. The frequency of duplicate mutant alleles recovered is consistent with a relatively small number of essential (EMB) genes with nonredundant functions during seed development. Other functions critical to seed development in Arabidopsis may be protected from deleterious mutations by extensive genome duplications.

MeSH Terms
Alleles Arabidopsis/genetics,physiology Chromosomes/genetics Crosses, Genetic Genome, Plant Models, Genetic Mutagenesis, Insertional Mutation Phenotype Plants, Genetically Modified Plasmids/metabolism Polymerase Chain Reaction Transformation, Genetic
Authors & Affiliations
16 authors, click to expand affiliations / ORCID
McElver J
Syngenta, Research Triangle Park, North Carolina 27709, USA.
Tzafrir I
Aux G
Rogers R
Ashby C
Smith K
Thomas C
Schetter A
Zhou Q
Cushman M A
Tossberg J
Nickle T
Levin J Z
Law M
Meinke D
Patton D
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/159.4.1751
Published
2001-12-00
Pages
1751-63
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1461914
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/159.4.1751 PMC Full Text View journal details
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