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PMID: 11230125 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Arabidopsis glucosidase I mutants reveal a critical role of N-glycan trimming in seed development.

The EMBO journal ·Vol. 20 ·No. 5 ·2001-03-01 ·Pages 1010-9

Boisson M, Gomord V, Audran C, Berger N, Dubreucq B, Granier F, Lerouge P, Faye L, Caboche M, Lepiniec L

Abstract

Glycoproteins with asparagine-linked (N-linked) glycans occur in all eukaryotic cells. The function of their glycan moieties is one of the central problems in contemporary cell biology. N-glycosylation may modify physicochemical and biological protein properties such as conformation, degradation, intracellular sorting or secretion. We have isolated and characterized two allelic Arabidopsis mutants, gcs1-1 and gcs1-2, which produce abnormal shrunken seeds, blocked at the heart stage of development. The mutant seeds accumulate a low level of storage proteins, have no typical protein bodies, display abnormal cell enlargement and show occasional cell wall disruptions. The mutated gene has been cloned by T-DNA tagging. It codes for a protein homologous to animal and yeast alpha-glucosidase I, an enzyme that controls the first committed step for N-glycan trimming. Biochemical analyses have confirmed that trimming of the alpha1,2- linked glucosyl residue constitutive of the N-glycan precursor is blocked in this mutant. These results demonstrate the importance of N-glycan trimming for the accumulation of seed storage proteins, the formation of protein bodies, cell differentiation and embryo development.

MeSH Terms
Alleles Amino Acid Sequence Arabidopsis/embryology,enzymology,genetics,ultrastructure Cell Differentiation Cloning, Molecular DNA, Bacterial/genetics Gene Expression Profiling Gene Expression Regulation, Plant Genes, Essential/genetics Genetic Complementation Test Glycosylation Histocytochemistry Immunoblotting Microscopy, Electron Molecular Sequence Data Mutation/genetics Phenotype Polysaccharides/chemistry,metabolism RNA, Messenger/analysis,genetics Seeds/embryology,enzymology,genetics,ultrastructure Sequence Homology, Amino Acid Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization alpha-Glucosidases/chemistry,genetics,metabolism
Chemicals
DNA, Bacterial Polysaccharides RNA, Messenger T-DNA glucosidase I alpha-Glucosidases
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Boisson M
Laboratoire de Biologie des Semences, INRA-INAPG, Route de St-Cyr, 78026 Versailles, France.
Gomord V
Audran C
Berger N
Dubreucq B
Granier F
Lerouge P
Faye L
Caboche M
Lepiniec L
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/20.5.1010
Published
2001-03-01
Pages
1010-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC145462
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/20.5.1010 PMC Full Text View journal details
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