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PMID: 10634910 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The ROOT MERISTEMLESS1/CADMIUM SENSITIVE2 gene defines a glutathione-dependent pathway involved in initiation and maintenance of cell division during postembryonic root development.

The Plant cell ·Vol. 12 ·No. 1 ·2000-01-00 ·Pages 97-110

Vernoux T, Wilson RC, Seeley KA, Reichheld JP, Muroy S, Brown S, Maughan SC, Cobbett CS, Van Montagu M, Inzé D, May MJ, Sung ZR

Abstract

Activation of cell division in the root apical meristem after germination is essential for postembryonic root development. Arabidopsis plants homozygous for a mutation in the ROOT MERISTEMLESS1 (RML1) gene are unable to establish an active postembryonic meristem in the root apex. This mutation abolishes cell division in the root but not in the shoot. We report the molecular cloning of the RML1 gene, which encodes the first enzyme of glutathione (GSH) biosynthesis, gamma-glutamylcysteine synthetase, and which is allelic to CADMIUM SENSITIVE2. The phenotype of the rml1 mutant, which was also evident in the roots of wild-type Arabidopsis and tobacco treated with an inhibitor of GSH biosynthesis, could be relieved by applying GSH to rml1 seedlings. By using a synchronized tobacco cell suspension culture, we showed that the G(1)-to-S phase transition requires an adequate level of GSH. These observations suggest the existence of a GSH-dependent developmental pathway essential for initiation and maintenance of cell division during postembryonic root development.

MeSH Terms

Alleles Amino Acid Sequence Arabidopsis/genetics,growth & development,metabolism Base Sequence Cell Division Cloning, Molecular DNA, Plant/genetics Genes, Plant Glutamate-Cysteine Ligase/genetics,metabolism Glutathione/metabolism Molecular Sequence Data Mutation Phenotype Plant Development Plant Roots/cytology,growth & development,metabolism Plants/genetics,metabolism Plants, Toxic Sequence Homology, Amino Acid Tobacco/genetics,growth & development,metabolism

Chemicals

DNA, Plant Glutamate-Cysteine Ligase Glutathione

Authors & Affiliations

12 authors, click to expand affiliations / ORCID

Vernoux T

Universiteit Gent, K.L. Ledeganckstraat 35, B-9000, Gent, Belgium.

Wilson R C

Seeley K A

Reichheld J P

Muroy S

Brown S

Maughan S C

Cobbett C S

Van Montagu M

Inzé D

May M J

Sung Z R

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Article Info

Journal

The Plant cell

Abbr.

Plant Cell

ISSN

1040-4651

DOI

10.1105/tpc.12.1.97

Published

2000-01-00

Pages

97-110

Language

English

Region

England

NLM ID

9208688

PMCID

PMC140217

Subset

External Links

PubMed source DOI: 10.1105/tpc.12.1.97 PMC Full Text View journal details

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The ROOT MERISTEMLESS1/CADMIUM SENSITIVE2 gene defines a glutathione-dependent pathway involved in initiation and maintenance of cell division during postembryonic root development.

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