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PMID: 11725943 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A catalytic subunit of the sugar beet protein kinase CK2 is induced by salt stress and increases NaCl tolerance in Saccharomyces cerevisiae.

Plant molecular biology ·Vol. 47 ·No. 5 ·2001-11-00 ·Pages 571-9

Kanhonou R, Serrano R, Palau RR

Abstract

Salinity is an important limiting factor in plant growth and development. We have cloned a catalytic subunit of the sugar beet protein kinase CK2 (BvCKA2) by functional expression in yeast of a NaCl-induced cDNA library. BvCKA2 was able to increase the yeast tolerance to NaCl and to functionally complement the cka1 cka2 yeast double mutant upon over-expression. Southern blot analysis indicated that, in sugar beet, the BCKA2 gene is a member of a multigene family. The mRNA levels of BvCKA2 were up-regulated in response to NaCl stress which suggests that protein kinase CK2 may be involved in the plant response to salt stress.

MeSH Terms
Adaptation, Physiological/genetics Amino Acid Sequence Beta vulgaris/enzymology,genetics Blotting, Southern Casein Kinase II Catalytic Domain Cell Division/drug effects,genetics DNA, Complementary/chemistry,genetics,isolation & purification DNA, Plant/genetics Gene Expression Regulation, Enzymologic/drug effects Gene Expression Regulation, Plant/drug effects Gene Library Genetic Complementation Test Molecular Sequence Data Mutation Plant Leaves/enzymology,genetics Potassium/metabolism Protein Serine-Threonine Kinases/genetics Saccharomyces cerevisiae/drug effects,genetics,metabolism Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid Sodium/metabolism Sodium Chloride/pharmacology
Chemicals
DNA, Complementary DNA, Plant Sodium Chloride Sodium Casein Kinase II Protein Serine-Threonine Kinases Potassium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kanhonou R
Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-CSIC, Spain.
Serrano R
Palau R R
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1023/a:1012227913356
Published
2001-11-00
Pages
571-9
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1023/a:1012227913356 View journal details
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