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PMID: 10634915 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Osmotic stress induces rapid activation of a salicylic acid-induced protein kinase and a homolog of protein kinase ASK1 in tobacco cells.

The Plant cell ·Vol. 12 ·No. 1 ·2000-01-00 ·Pages 165-78

Mikołajczyk M, Awotunde OS, Muszyńska G, Klessig DF, Dobrowolska G

Abstract

In tobacco cells, osmotic stress induced the rapid activation of two protein kinases that phosphorylate myelin basic protein. Immunological studies demonstrated that the 48-kD kinase is the salicylic acid-induced protein kinase (SIPK), a member of the mitogen-activated protein kinase family. SIPK was activated 5 to 10 min after the cells were exposed to osmotic stresses, and its activity persisted for approximately 30 min. In contrast, the 42-kD kinase was activated within 1 min after osmotic stress, and its activity was maintained for approximately 2 hr. Moreover, in addition to myelin basic protein, the 42-kD kinase phosphorylated casein and two transcription factors, c-Jun and ATF-2. This latter enzyme was inactivated by a serine/threonine-specific phosphatase but, unlike SIPK, was not affected by a tyrosine-specific phosphatase. After the 42-kD kinase was purified to apparent homogeneity, tryptic peptide analysis indicated that it is a homolog of Arabidopsis serine/threonine kinase1 (ASK1).

MeSH Terms
Amino Acid Sequence Arabidopsis/enzymology,genetics Arabidopsis Proteins Enzyme Activation Immunoblotting Mitogen-Activated Protein Kinases/chemistry,genetics,metabolism Molecular Sequence Data Molecular Weight Osmotic Pressure Phosphorylation Plant Proteins/chemistry,genetics,metabolism Plants, Toxic Protein Serine-Threonine Kinases/chemistry,genetics,metabolism Sequence Homology, Amino Acid Substrate Specificity Tobacco/enzymology,genetics
Chemicals
ASK1 protein, Arabidopsis Arabidopsis Proteins Plant Proteins Protein Serine-Threonine Kinases Mitogen-Activated Protein Kinases SA-induced protein kinase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Mikołajczyk M
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, ul. Pawińskiego 5a, 02-106 Warsaw, Poland.
Awotunde O S
Muszyńska G
Klessig D F
Dobrowolska G
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
Published
2000-01-00
Pages
165-78
Language
English
Region
England
NLM ID
9208688
PMCID
PMC149182
Subset
IM
Corrections
ErratumIn
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External Links
PubMed source PMC Full Text View journal details
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