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PMID: 10069836 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Antisense expression of the CK2 alpha-subunit gene in Arabidopsis. Effects on light-regulated gene expression and plant growth.

Plant physiology ·Vol. 119 ·No. 3 ·1999-03-00 ·Pages 989-1000

Lee Y, Lloyd AM, Roux SJ

Abstract

The protein kinase CK2 (formerly casein kinase II) is thought to be involved in light-regulated gene expression in plants because of its ability to phosphorylate transcription factors that bind to the promoter regions of light-regulated genes in vitro. To address this possibility in vivo and to learn more about the potential physiological roles of CK2 in plants, we transformed Arabidopsis with an antisense construct of the CK2 alpha-subunit gene and investigated both morphological and molecular phenotypes. Antisense transformants had a smaller adult leaf size and showed increased expression of chs in darkness and of cab and rbcS after red-light treatment. The latter molecular phenotype implied that CK2 might serve as one of several negative and quantitative effectors in light-regulated gene expression. The possible mechanism of CK2 action and its involvement in the phytochrome signal transduction pathway are discussed.

Keywords
Non-programmatic
MeSH Terms
Arabidopsis/enzymology,genetics,growth & development Base Sequence CDC2 Protein Kinase/genetics,metabolism Casein Kinase II DNA, Antisense/genetics Gene Expression Regulation, Enzymologic/radiation effects Gene Expression Regulation, Plant/radiation effects Genes, Plant Light Plant Leaves/growth & development Plants, Genetically Modified Protein Serine-Threonine Kinases/genetics RNA, Messenger/genetics,metabolism RNA, Plant/genetics,metabolism Transformation, Genetic
Chemicals
DNA, Antisense RNA, Messenger RNA, Plant Casein Kinase II Protein Serine-Threonine Kinases CDC2 Protein Kinase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lee Y
Department of Botany and Institute for Cellular and Molecular Biology, The University of Texas, Austin, Texas 78713, USA.
Lloyd A M
Roux S J
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.119.3.989
Published
1999-03-00
Pages
989-1000
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC32112
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.119.3.989 PMC Full Text View journal details
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