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PMID: 1840925 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The Arabidopsis functional homolog of the p34cdc2 protein kinase.

The Plant cell ·Vol. 3 ·No. 5 ·1991-05-00 ·Pages 531-40

Ferreira PC, Hemerly AS, Villarroel R, Van Montagu M, Inzé D

Abstract

The p34cdc2 protein kinase is a key component of the eukaryotic cell cycle, which is required for G1 to S-phase transition and for entry into mitosis. Using a 380-base pair DNA fragment obtained by polymerase chain reaction amplification from an Arabidopsis thaliana flower cDNA library as a probe, we isolated and sequenced a cdc2-homologous cDNA from Arabidopsis. The encoded polypeptide has extensive homology with cdc2-like kinases. Furthermore, when expressed in a CDC28ts Saccharomyces strain, it partially restores the capacity to grow at 36 degrees C, indicating that the plant cDNA is a functional homolog of the p34cdc2 kinase. Genomic hybridization demonstrated that there is one copy of the cdc2 gene per Arabidopsis haploid genome. Using RNA gel blot analysis, we found that cdc2 mRNA is present in all plant organs.

Related Genes
cdc2
MeSH Terms
Amino Acid Sequence Arabidopsis/embryology,genetics Base Sequence CDC2 Protein Kinase/genetics,metabolism Cell Division/genetics Cloning, Molecular DNA Genetic Complementation Test Molecular Sequence Data Saccharomyces cerevisiae/genetics Sequence Homology, Amino Acid
Chemicals
DNA CDC2 Protein Kinase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Ferreira P C
Laboratorium voor Genetica, Rijksuniversiteit Gent, Belgium.
Hemerly A S
Villarroel R
Van Montagu M
Inzé D
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.3.5.531
Published
1991-05-00
Pages
531-40
Language
English
Region
England
NLM ID
9208688
PMCID
PMC160020
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.3.5.531 PMC Full Text View journal details
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