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PMID: 11597999 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Calcium-dependent protein kinases play an essential role in a plant defence response.

The EMBO journal ·Vol. 20 ·No. 20 ·2001-10-15 ·Pages 5556-67

Romeis T, Ludwig AA, Martin R, Jones JD

Abstract

Calcium-dependent protein kinases (CDPKs) comprise a large family of serine/threonine kinases in plants and protozoans. We isolated two related CDPK cDNAs (NtCDPK2 and NtCDPK3) from Nicotiana tabacum. These CDPK transcripts are elevated after race-specific defence elicitation and hypo-osmotic stress. Transiently expressed myc-epitope-tagged NtCDPK2 in Nicotiana benthamiana and N.tabacum leaves showed a rapid transient interconversion to an activated form after elicitation and hypo-osmotic stress. The Avr9 race-specific elicitor caused a more pronounced and sustained response. This transition is due to phosphorylation of the CDPK. Immuno complex kinase assays with epitope-tagged NtCDPK2 showed that stress-induced phosphorylation and interconversion of NtCDPK2 correlates with an increase in enzymatic activity. The function of NtCDPK2 in plant defence was investigated by employing virus-induced gene silencing (VIGS) in N.benthamiana. CDPK-silenced plants showed a reduced and delayed hypersensitive response after race-specific elicitation in a gene-for-gene interaction, and lacked an accompanying wilting phenotype. Silencing correlated with loss of CDPK mRNA, whereas mRNA accumulation of mitogen-activated protein kinase WIPK remained unaltered.

MeSH Terms
Amino Acid Sequence Calcium-Binding Proteins/genetics,physiology Cells, Cultured/enzymology Cloning, Molecular DNA, Complementary/genetics,isolation & purification Enzyme Induction Fungal Proteins/genetics,physiology Gene Silencing MAP Kinase Signaling System Membrane Glycoproteins/genetics,physiology Mitogen-Activated Protein Kinases/analysis Molecular Sequence Data Multigene Family Osmotic Pressure Phenotype Phosphorylation Plant Diseases/genetics,virology Plant Leaves/enzymology Plant Proteins/genetics,physiology Potexvirus/physiology Protein Kinases/genetics,physiology Protein Processing, Post-Translational Protozoan Proteins RNA, Messenger/genetics RNA, Plant/genetics Receptor Cross-Talk Sequence Alignment Sequence Homology, Amino Acid Signal Transduction Tobacco/cytology,enzymology,genetics,physiology
Chemicals
AVR4 protein, Cladosporium fulvum Calcium-Binding Proteins Cf protein, Lycopersicon esculentum DNA, Complementary Fungal Proteins Membrane Glycoproteins Plant Proteins Protozoan Proteins RNA, Messenger RNA, Plant AVR9 protein, Cladosporium fulvum Protein Kinases CDPK3 protein, Plasmodium falciparum Mitogen-Activated Protein Kinases WIPK protein, Nicotiana tabacum
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Romeis T
The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, Norfolk, UK. romeis@mpiz-koeln.mpg.de
Ludwig A A
Martin R
Jones J D
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/20.20.5556
Email
romeis@mpiz-koeln.mpg.de
Published
2001-10-15
Pages
5556-67
Language
English
Region
England
NLM ID
8208664
PMCID
PMC125278
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/20.20.5556 PMC Full Text View journal details
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