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PMID: 8978683 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

A novel chloride channel in Vicia faba guard cell vacuoles activated by the serine/threonine kinase, CDPK.

The EMBO journal ·Vol. 15 ·No. 23 ·1996-12-02 ·Pages 6564-74

Pei ZM, Ward JM, Harper JF, Schroeder JI

Abstract

Calcium-Dependent Protein Kinases (CDPKs) in higher plants contain a C-terminal calmodulin-like regulatory domain. Little is known regarding physiological CDPK targets. Both kinase activity and multiple Ca2+-dependent signaling pathways have been implicated in the control of stomatal guard cell movements. To determine whether CDPK or other protein kinases could have a role in guard cell signaling, purified and recombinant kinases were applied to Vicia faba guard cell vacuoles during patch-clamp experiments. CDPK activated novel vacuolar chloride (VCL) and malate conductances in guard cells. Activation was dependent on both Ca2+ and ATP. Furthermore, VCL activation occurred in the absence of Ca2+ using a Ca2+-independent, constitutively active, CDPK* mutant. Protein kinase A showed weaker activation (22% as compared with CDPK). Current reversals in whole vacuole recordings shifted with the Nernst potential for Cl-and vanished in glutamate. Single channel recordings showed a CDPK-activated 34 +/- 5 pS Cl- channel. VCL channels were activated at physiological potentials enabling Cl- uptake into vacuoles. VCL channels may provide a previously unidentified, but necessary, pathway for anion uptake into vacuoles required for stomatal opening. CDPK-activated VCL currents were also observed in red beet vacuoles suggesting that these channels may provide a more general mechanism for kinase-dependent anion uptake.

Keywords
Non-programmatic
MeSH Terms
Adenosine Triphosphate/metabolism Calcium/metabolism Cell Movement Chloride Channels/physiology Cytosol/metabolism Fabaceae/cytology,physiology Kinetics Membrane Potentials Patch-Clamp Techniques Plants, Medicinal Protein Serine-Threonine Kinases/metabolism Species Specificity Vacuoles/physiology,ultrastructure Vegetables
Chemicals
Chloride Channels Adenosine Triphosphate Protein Serine-Threonine Kinases Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Pei Z M
Department of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla 92093-0116, USA.
Ward J M
Harper J F
Schroeder J I
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1996-12-02
Pages
6564-74
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452481
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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