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PMID: 11483958 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The role of Drosophila CID in kinetochore formation, cell-cycle progression and heterochromatin interactions.

Nature cell biology ·Vol. 3 ·No. 8 ·2001-08-00 ·Pages 730-9

Blower MD, Karpen GH

Abstract

Centromere function requires the coordination of many processes including kinetochore assembly, sister chromatid cohesion, spindle attachment and chromosome movement. Here we show that CID, the Drosophila homologue of the CENP-A centromere-specific H3-like proteins, colocalizes with molecular-genetically defined functional centromeres in minichromosomes. Injection of CID antibodies into early embryos, as well as RNA interference in tissue-culture cells, showed that CID is required for several mitotic processes. Deconvolution fluorescence microscopy showed that CID chromatin is physically separate from proteins involved in sister cohesion (MEI-S332), centric condensation (PROD), kinetochore function (ROD, ZW10 and BUB1) and heterochromatin structure (HP1). CID localization is unaffected by mutations in mei-S332, Su(var)2-5 (HP1), prod or polo. Furthermore, the localization of POLO, CENP-meta, ROD, BUB1 and MEI-S332, but not PROD or HP1, depends on the presence of functional CID. We conclude that the centromere and flanking heterochromatin are physically and functionally separable protein domains that are required for different inheritance functions, and that CID is required for normal kinetochore formation and function, as well as cell-cycle progression.

MeSH Terms
Animals Antibodies/pharmacology Autoantigens Cell Cycle/genetics Cell Cycle Proteins/genetics,metabolism Cells, Cultured Centromere Protein A Chromosomal Proteins, Non-Histone/genetics,metabolism Chromosome Segregation/genetics DNA/metabolism DNA-Binding Proteins Drosophila Proteins Drosophila melanogaster/genetics,metabolism Embryo, Nonmammalian/cytology,drug effects,metabolism Heterochromatin/genetics Histones/genetics,immunology,metabolism Insect Proteins/genetics,metabolism Interphase/genetics Kinetochores/metabolism Microtubule-Associated Proteins/genetics,metabolism Mutation/physiology Protein Kinases/genetics,metabolism Protein Serine-Threonine Kinases/genetics,metabolism
Chemicals
Antibodies Autoantigens Cell Cycle Proteins Centromere Protein A Chromosomal Proteins, Non-Histone Cid protein, Drosophila DNA-Binding Proteins Drosophila Proteins Heterochromatin Histones Insect Proteins Microtubule-Associated Proteins cmet protein, Drosophila mei-S332 protein, Drosophila prod protein, Drosophila Zw10 protein, Drosophila DNA Protein Kinases polo protein, Drosophila Bub1 spindle checkpoint protein Protein Serine-Threonine Kinases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Blower M D
Molecular and Cell Biology Laboratories, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037, USA.
Karpen G H
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Article Info
Journal
Nature cell biology
Abbr.
Nat Cell Biol
ISSN
1465-7392
DOI
10.1038/35087045
Published
2001-08-00
Pages
730-9
Language
English
Region
England
NLM ID
100890575
PMCID
PMC3229202
Subset
IM
Grants
NIGMS NIH HHS · R01 GM066272 · United States
NIGMS NIH HHS · R01 GM066272-05 · United States
External Links
PubMed source DOI: 10.1038/35087045 PMC Full Text View journal details
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