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PMID: 7664339 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Localization of centromere function in a Drosophila minichromosome.

Cell ·Vol. 82 ·No. 4 ·1995-08-25 ·Pages 599-609

Murphy TD, Karpen GH

Abstract

The DNA elements responsible for centromere activity in a metazoan have been localized using the Drosophila minichromosome Dp1187. Deleted minichromosomes were generated by irradiation mutagenesis, and their molecular structures were determined by pulsed-field Southern blot analysis. Analyses of the transmission behavior of Dp1187 derivatives localized sequences necessary for chromosome inheritance within the centric heterochromatin. The essential core of the centromere is contained within a 220 kb region that includes significant amounts of complex DNA. Completely normal inheritance also requires approximately 200 kb on either side of the essential core. This flanking DNA predominantly contains highly repeated sequences, and the amount required for normal transmission differs among division types and between the sexes. We propose that the essential core is the site of kinetochore formation and that flanking DNA provides two functions: sister chromatid cohesion and indirect assistance in kinetochore formation or function.

MeSH Terms
Animals Centromere/genetics,physiology Chromosome Deletion Chromosomes/genetics,ultrastructure DNA/genetics Drosophila/genetics Female Genes, Insect Kinetochores/physiology Male Models, Genetic Schizosaccharomyces/genetics
Chemicals
DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Murphy T D
Molecular Biology and Virology Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037, USA.
Karpen G H
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
0092-8674
DOI
10.1016/0092-8674(95)90032-2
Published
1995-08-25
Pages
599-609
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC3209481
Subset
IM
Grants
NHGRI NIH HHS · R01 HG000747 · United States
NHGRI NIH HHS · R01 HG00747 · United States
External Links
PubMed source DOI: 10.1016/0092-8674(95)90032-2 PMC Full Text View journal details
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