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PMID: 1334894 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Analysis of subtelomeric heterochromatin in the Drosophila minichromosome Dp1187 by single P element insertional mutagenesis.

Genetics ·Vol. 132 ·No. 3 ·1992-11-00 ·Pages 737-53

Karpen GH, Spradling AC

Abstract

We investigated whether single P element insertional mutagenesis could be used to analyze heterochromatin within the Drosophila minichromosome Dp1187. Forty-five insertions of the P[lacZ,rosy+] element onto Dp1187 (recovered among 7,825 transpositions) were highly clustered. None was recovered in centromeric heterochromatin, but 39 occurred about 40 kb from the distal telomere within a 4.7-kb hotspot containing tandem copies of a novel 1.8-kb repetitive DNA sequence. The DNA within and distal to this region lacked essential genes and displayed several other properties characteristic of heterochromatin. The rosy+ genes within the inserted transposons were inhibited by position-effect variegation, and the subtelomeric region was underrepresented in polytene salivary gland cells. These experiments demonstrated that P elements preferentially transpose into a small subset of heterochromatic sites, providing a versatile method for studying the structure and function of these chromosome regions. This approach revealed that a Drosophila chromosome contains a large region of subtelomeric heterochromatin with specific structural and genetic properties.

Related Genes
Sb ry
MeSH Terms
Animals Base Sequence Chromosomes/ultrastructure DNA Transposable Elements Drosophila/genetics Female Gene Expression Heterochromatin Male Molecular Sequence Data Mutagenesis, Insertional
Chemicals
DNA Transposable Elements Heterochromatin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Karpen G H
Howard Hughes Medical Institute Research Laboratories, Carnegie Institution of Washington, Department of Embryology, Baltimore, Maryland 21210.
Spradling A C
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/132.3.737
Published
1992-11-00
Pages
737-53
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1205211
Subset
IM
Grants
Howard Hughes Medical Institute · United States
NIGMS NIH HHS · GM27875 · United States
Databases
GENBANK
L03284, X62185, X62186, X62187, X62188, X62189, X62190, X67048, X67049, Z14148
External Links
PubMed source DOI: 10.1093/genetics/132.3.737 PMC Full Text View journal details
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