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PMID: 10893249 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

CENP-meta, an essential kinetochore kinesin required for the maintenance of metaphase chromosome alignment in Drosophila.

The Journal of cell biology ·Vol. 150 ·No. 1 ·2000-07-10 ·Pages 1-11

Yucel JK, Marszalek JD, McIntosh JR, Goldstein LS, Cleveland DW, Philp AV

Abstract

CENP-meta has been identified as an essential, kinesin-like motor protein in Drosophila. The 257-kD CENP-meta protein is most similar to the vertebrate kinetochore-associated kinesin-like protein CENP-E, and like CENP-E, is shown to be a component of centromeric/kinetochore regions of Drosophila chromosomes. However, unlike CENP-E, which leaves the centromere/kinetochore region at the end of anaphase A, the CENP-meta protein remains associated with the centromeric/kinetochore region of the chromosome during all stages of the Drosophila cell cycle. P-element-mediated disruption of the CENP-meta gene leads to late larval/pupal stage lethality with incomplete chromosome alignment at metaphase. Complete removal of CENP-meta from the female germline leads to lethality in early embryos resulting from defects in metaphase chromosome alignment. Real-time imaging of these mutants with GFP-labeled chromosomes demonstrates that CENP-meta is required for the maintenance of chromosomes at the metaphase plate, demonstrating that the functions required to establish and maintain chromosome congression have distinguishable requirements.

MeSH Terms
Animals Cell Cycle/genetics Cells, Cultured Centromere/metabolism Chromosomal Proteins, Non-Histone/genetics Chromosome Segregation/genetics Cloning, Molecular Drosophila Drosophila Proteins Female Gene Deletion Genes, Lethal Germ-Line Mutation Kinetochores/metabolism Metaphase/genetics Microtubule-Associated Proteins/genetics,metabolism Mitosis/genetics Molecular Motor Proteins/genetics,metabolism Molecular Sequence Data Mutagenesis, Insertional Sequence Homology, Amino Acid
Chemicals
Chromosomal Proteins, Non-Histone Drosophila Proteins Microtubule-Associated Proteins Molecular Motor Proteins cana protein, Drosophila centromere protein E cmet protein, Drosophila
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Yucel J K
Ludwig Institute for Cancer Research, University of California, San Diego, La Jolla, California 92093, USA.
Marszalek J D
McIntosh J R
Goldstein L S
Cleveland D W
Philp A V
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.150.1.1
Published
2000-07-10
Pages
1-11
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2185570
Subset
IM
Grants
NIGMS NIH HHS · GM29513 · United States
Databases
GENBANK
AF220353, AF220354
External Links
PubMed source DOI: 10.1083/jcb.150.1.1 PMC Full Text View journal details
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