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PMID: 9700166 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mitotic centromere-associated kinesin is important for anaphase chromosome segregation.

The Journal of cell biology ·Vol. 142 ·No. 3 ·1998-08-10 ·Pages 787-801

Maney T, Hunter AW, Wagenbach M, Wordeman L

Abstract

Mitotic centromere-associated kinesin (MCAK) is recruited to the centromere at prophase and remains centromere associated until after telophase. MCAK is a homodimer that is encoded by a single gene and has no associated subunits. A motorless version of MCAK that binds centromeres but not microtubules disrupts chromosome segregation during anaphase. Antisense-induced depletion of MCAK results in the same defect. MCAK overexpression induces centromere-independent bundling and eventual loss of spindle microtubule polymer suggesting that centromere-associated bundling and/or depolymerization activity is required for anaphase. Live cell imaging indicates that MCAK may be required to coordinate the onset of sister centromere separation.

MeSH Terms
Anaphase/physiology Animals CHO Cells Centromere/metabolism Chromosomes/genetics Cricetinae Kinesins/genetics,physiology Microtubules/metabolism Mitosis/genetics Spindle Apparatus/metabolism
Chemicals
mitotic centromere-associated kinesin, hamster Kinesins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Maney T
Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle, Washington 98195, USA.
Hunter A W
Wagenbach M
Wordeman L
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.142.3.787
Published
1998-08-10
Pages
787-801
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2148171
Subset
IM
Grants
NIGMS NIH HHS · GM53654A · United States
PHS HHS · T326M07270 · United States
External Links
PubMed source DOI: 10.1083/jcb.142.3.787 PMC Full Text View journal details
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