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PMID: 9334346 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The microtubule-dependent motor centromere-associated protein E (CENP-E) is an integral component of kinetochore corona fibers that link centromeres to spindle microtubules.

The Journal of cell biology ·Vol. 139 ·No. 2 ·1997-10-20 ·Pages 435-47

Yao X, Anderson KL, Cleveland DW

Abstract

Centromere-associated protein E (CENP-E) is a kinesin-related microtubule motor protein that is essential for chromosome congression during mitosis. Using immunoelectron microscopy, CENP-E is shown to be an integral component of the kinetochore corona fibers that tether centromeres to the spindle. Immediately upon nuclear envelope fragmentation, an associated plus end motor trafficks cytoplasmic CENP-E toward chromosomes along astral microtubules that enter the nuclear volume. Before or concurrently with initial lateral attachment of spindle microtubules, CENP-E targets to the outermost region of the developing kinetochores. After stable attachment, throughout chromosome congression, at metaphase, and throughout anaphase A, CENP-E is a constituent of the corona fibers, extending at least 50 nm away from the kinetochore outer plate and intertwining with spindle microtubules. In congressing chromosomes, CENP-E is preferentially associated with (or accessible at) the stretched, leading kinetochore known to provide the primary power for chromosome movement. Taken together, this evidence strongly supports a model in which CENP-E functions in congression to tether kinetochores to the disassembling microtubule plus ends.

MeSH Terms
Cell Cycle/physiology Cell Division Centromere/ultrastructure Chromosomal Proteins, Non-Histone/analysis Chromosomes, Human/ultrastructure HeLa Cells Humans Kinetochores/ultrastructure Microscopy, Immunoelectron Microtubules/drug effects,ultrastructure Models, Structural Nocodazole/pharmacology Nuclear Envelope/physiology,ultrastructure Spindle Apparatus/ultrastructure
Chemicals
Chromosomal Proteins, Non-Histone centromere protein E Nocodazole
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Yao X
Laboratory of Cell Biology, Ludwig Institute for Cancer Research, School of Medicine, University of California, La Jolla, CA 92093-0660, USA.
Anderson K L
Cleveland D W
References (60)
60 references, click to expand
  1. Mitotic forces control a cell-cycle checkpoint.
    Nature. 1995 Feb 16;373(6515):630-2 PMID: 7854422
  2. Purification of the centromere-specific protein CENP-A and demonstration that it is a distinctive histone.
    Proc Natl Acad Sci U S A. 1991 May 1;88(9):3734-8 PMID: 2023923
  3. Mitotic HeLa cells contain a CENP-E-associated minus end-directed microtubule motor.
    EMBO J. 1995 Mar 1;14(5):918-26 PMID: 7889940
  4. Kinetochore chemistry is sensitive to tension and may link mitotic forces to a cell cycle checkpoint.
    J Cell Biol. 1995 Aug;130(4):929-39 PMID: 7642708
  5. The checkpoint delaying anaphase in response to chromosome monoorientation is mediated by an inhibitory signal produced by unattached kinetochores.
    J Cell Biol. 1995 Aug;130(4):941-8 PMID: 7642709
  6. The centromere: hub of chromosomal activities.
    Science. 1995 Dec 8;270(5242):1591-4 PMID: 7502067
  7. XKCM1: a Xenopus kinesin-related protein that regulates microtubule dynamics during mitotic spindle assembly.
    Cell. 1996 Jan 12;84(1):37-47 PMID: 8548824
  8. Modulation of CENP-E organization at kinetochores by spindle microtubule attachment.
    Cell Motil Cytoskeleton. 1996;35(2):121-33 PMID: 8894282
  9. Kinetochores moving away from their associated pole do not exert a significant pushing force on the chromosome.
    J Cell Biol. 1996 Oct;135(2):315-27 PMID: 8896591
  10. Two different microtubule-based motor activities with opposite polarities in kinetochores.
    Nature. 1991 May 16;351(6323):206-11 PMID: 2041567
  11. Chemical subdomains within the kinetochore domain of isolated CHO mitotic chromosomes.
    J Cell Biol. 1991 Jul;114(2):285-94 PMID: 1830054
  12. Structural and mechanical control of mitotic progression.
    Cold Spring Harb Symp Quant Biol. 1991;56:613-9 PMID: 1819511
  13. CENP-C, an autoantigen in scleroderma, is a component of the human inner kinetochore plate.
    Cell. 1992 Jul 10;70(1):115-25 PMID: 1339310
  14. CENP-E is a putative kinetochore motor that accumulates just before mitosis.
    Nature. 1992 Oct 8;359(6395):536-9 PMID: 1406971
  15. Structure of the colcemid-treated PtK1 kinetochore outer plate as determined by high voltage electron microscopic tomography.
    J Cell Biol. 1993 Jan;120(2):301-12 PMID: 8421050
  16. Directional instability of kinetochore motility during chromosome congression and segregation in mitotic newt lung cells: a push-pull mechanism.
    J Cell Biol. 1993 Aug;122(4):859-75 PMID: 8349735
  17. Cytoplasmic dynein plays a role in mammalian mitotic spindle formation.
    J Cell Biol. 1993 Nov;123(4):849-58 PMID: 8227145
  18. Disruption of mitotic spindle orientation in a yeast dynein mutant.
    Proc Natl Acad Sci U S A. 1993 Nov 1;90(21):10096-100 PMID: 8234262
  19. Cytoplasmic dynein is required for normal nuclear segregation in yeast.
    Proc Natl Acad Sci U S A. 1993 Dec 1;90(23):11172-6 PMID: 8248224
  20. Cyclin-like accumulation and loss of the putative kinetochore motor CENP-E results from coupling continuous synthesis with specific degradation at the end of mitosis.
    J Cell Biol. 1994 Jun;125(6):1303-12 PMID: 8207059
  21. Mitotic regulation of microtubule cross-linking activity of CENP-E kinetochore protein.
    Science. 1994 Jul 15;265(5170):394-8 PMID: 8023161
  22. Anaphase onset in vertebrate somatic cells is controlled by a checkpoint that monitors sister kinetochore attachment to the spindle.
    J Cell Biol. 1994 Dec;127(5):1301-10 PMID: 7962091
  23. Minus-end-directed motion of kinesin-coated microspheres driven by microtubule depolymerization.
    Nature. 1995 Jan 12;373(6510):161-4 PMID: 7816099
  24. Aberrantly segregating centromeres activate the spindle assembly checkpoint in budding yeast.
    J Cell Biol. 1996 Apr;133(1):75-84 PMID: 8601615
  25. Molecular characterization of the 50-kD subunit of dynactin reveals function for the complex in chromosome alignment and spindle organization during mitosis.
    J Cell Biol. 1996 Feb;132(4):617-33 PMID: 8647893
  26. Antibodies to the kinesin motor domain and CENP-E inhibit microtubule depolymerization-dependent motion of chromosomes in vitro.
    J Cell Biol. 1995 Jan;128(1-2):107-15 PMID: 7822408
  27. Biochemical characterization of ezrin-actin interaction.
    J Biol Chem. 1996 Mar 22;271(12):7224-9 PMID: 8636161
  28. Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts.
    Nature. 1996 Aug 1;382(6590):420-5 PMID: 8684481
  29. The kinesin-like protein CENP-E is kinetochore-associated throughout poleward chromosome segregation during anaphase-A.
    J Cell Sci. 1996 May;109 ( Pt 5):961-9 PMID: 8743943
  30. Association of spindle assembly checkpoint component XMAD2 with unattached kinetochores.
    Science. 1996 Oct 11;274(5285):242-6 PMID: 8824188
  31. Identification of a human mitotic checkpoint gene: hsMAD2.
    Science. 1996 Oct 11;274(5285):246-8 PMID: 8824189
  32. Identification and partial characterization of mitotic centromere-associated kinesin, a kinesin-related protein that associates with centromeres during mitosis.
    J Cell Biol. 1995 Jan;128(1-2):95-104 PMID: 7822426
  33. Opposing motor activities are required for the organization of the mammalian mitotic spindle pole.
    J Cell Biol. 1996 Oct;135(2):399-414 PMID: 8896597
  34. A complex of NuMA and cytoplasmic dynein is essential for mitotic spindle assembly.
    Cell. 1996 Nov 1;87(3):447-58 PMID: 8898198
  35. The spindle assembly checkpoint.
    Curr Opin Cell Biol. 1996 Dec;8(6):773-80 PMID: 8939672
  36. Oscillating mitotic newt lung cell kinetochores are, on average, under tension and rarely push.
    J Cell Sci. 1996 Dec;109 ( Pt 12):2823-31 PMID: 9013330
  37. CENP-E is an essential kinetochore motor in maturing oocytes and is masked during mos-dependent, cell cycle arrest at metaphase II.
    Proc Natl Acad Sci U S A. 1997 Aug 19;94(17):9165-70 PMID: 9256453
  38. CENP-E is a plus end-directed kinetochore motor required for metaphase chromosome alignment.
    Cell. 1997 Oct 31;91(3):357-66 PMID: 9363944
  39. The structure of the kinetochore in meiosis and mitosis in Urechis eggs.
    Exp Cell Res. 1965 Sep;39(2):643-57 PMID: 5891318
  40. The fine structure of the kinetochore of a mammalian cell in vitro.
    Chromosoma. 1966;19(1):28-43 PMID: 5912064
  41. The ultrastructure and spatial organization of the metaphase kinetochore in mitotic rat cells.
    J Ultrastruct Res. 1967 Jul;19(1):19-44 PMID: 5339062
  42. Holocentric chromosomes in Oncopeltus: kinetochore plates are present in mitosis but absent in meiosis.
    Chromosoma. 1972;37(2):177-92 PMID: 5043259
  43. Light and electron microscopy of rat kangaroo cells in mitosis. I. Formation and breakdown of the mitotic apparatus.
    Chromosoma. 1973;40(1):43-82 PMID: 4344486
  44. Structure of the mammalian kinetochore.
    Chromosoma. 1981;82(2):153-70 PMID: 7227036
  45. Higher order metaphase chromosome structure: evidence for metalloprotein interactions.
    Cell. 1982 May;29(1):171-81 PMID: 7105181
  46. Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line.
    J Cell Biol. 1982 Jun;93(3):576-82 PMID: 6811596
  47. The formation, structure, and composition of the mammalian kinetochore and kinetochore fiber.
    Int Rev Cytol. 1982;79:1-58 PMID: 6185450
  48. Properties of the kinetochore in vitro. I. Microtubule nucleation and tubulin binding.
    J Cell Biol. 1985 Sep;101(3):755-65 PMID: 4030893
  49. Organization within the mammalian kinetochore.
    Chromosoma. 1986;93(6):515-20 PMID: 3731942
  50. The molecular structure of adrenal medulla kinesin.
    Cell Motil Cytoskeleton. 1989;12(4):264-72 PMID: 2497994
  51. The motor for poleward chromosome movement in anaphase is in or near the kinetochore.
    J Cell Biol. 1989 Nov;109(5):2245-55 PMID: 2808528
  52. Kinetochores are transported poleward along a single astral microtubule during chromosome attachment to the spindle in newt lung cells.
    J Cell Biol. 1990 Jan;110(1):81-95 PMID: 2295685
  53. Cytoplasmic dynein is localized to kinetochores during mitosis.
    Nature. 1990 May 17;345(6272):263-5 PMID: 2139717
  54. Localization of cytoplasmic dynein to mitotic spindles and kinetochores.
    Nature. 1990 May 17;345(6272):266-8 PMID: 2139718
  55. CENP-B: a major human centromere protein located beneath the kinetochore.
    J Cell Biol. 1990 May;110(5):1475-88 PMID: 2335558
  56. Kinetochores capture astral microtubules during chromosome attachment to the mitotic spindle: direct visualization in live newt lung cells.
    J Cell Biol. 1990 Sep;111(3):1039-45 PMID: 2391359
  57. Identification of novel centromere/kinetochore-associated proteins using monoclonal antibodies generated against human mitotic chromosome scaffolds.
    J Cell Biol. 1991 Mar;112(6):1083-97 PMID: 1999466
  58. Taxol-induced microtubule asters in mitotic extracts of Xenopus eggs: requirement for phosphorylated factors and cytoplasmic dynein.
    J Cell Biol. 1991 Mar;112(6):1177-87 PMID: 1671864
  59. CENP-E, a novel human centromere-associated protein required for progression from metaphase to anaphase.
    EMBO J. 1991 May;10(5):1245-54 PMID: 2022189
  60. Saccharomyces cerevisiae kinesin- and dynein-related proteins required for anaphase chromosome segregation.
    J Cell Biol. 1995 Feb;128(4):617-24 PMID: 7860634
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.139.2.435
Published
1997-10-20
Pages
435-47
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2139792
Subset
IM
Grants
NIGMS NIH HHS · GM 29513 · United States
External Links
PubMed source DOI: 10.1083/jcb.139.2.435 PMC Full Text View journal details
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