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PMID: 2023923 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Purification of the centromere-specific protein CENP-A and demonstration that it is a distinctive histone.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 9 ·1991-05-01 ·Pages 3734-8

Palmer DK, O'Day K, Trong HL, Charbonneau H, Margolis RL

Abstract

CENP-A, a centromere-specific 17-kDa protein, has histone-like properties. However, in contrast to the common somatic histones, CENP-A is quantitatively retained in bull spermatozoa, and we have exploited this fact to purify CENP-A to apparent homogeneity. Partial sequence analysis of the purified protein indicates that CENP-A is a distinctive gene product. Some CENP-A sequences are highly similar to regions of histone H3. Other segments of CENP-A are not related to H3 or any other histone. These unrelated segments are presumably involved in localizing CENP-A to centromeric DNA or in centromere-specific functions of CENP-A.

MeSH Terms
Amino Acid Sequence Animals Autoantigens Cattle Cell Nucleus/chemistry Centromere/chemistry Centromere Protein A Chromosomal Proteins, Non-Histone/chemistry,isolation & purification Histones/chemistry,isolation & purification Male Molecular Sequence Data Molecular Weight Nuclear Proteins/chemistry Peptide Mapping Spermatozoa/chemistry
Chemicals
Autoantigens Centromere Protein A Chromosomal Proteins, Non-Histone Histones Nuclear Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Palmer D K
Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
O'Day K
Trong H L
Charbonneau H
Margolis R L
References (29)
29 references, click to expand
  1. The cyclic tyrosination/detyrosination of alpha tubulin.
    Methods Cell Biol. 1982;24:235-55 PMID: 7098991
  2. The centromere specific histone CENP-A is selectively retained in discrete foci in mammalian sperm nuclei.
    Chromosoma. 1990 Dec;100(1):32-6 PMID: 2101350
  3. Histone fractionation by high-performance liquid chromatography.
    Anal Biochem. 1983 Feb 15;129(1):132-44 PMID: 6859518
  4. The kinetochore is part of the metaphase chromosome scaffold.
    J Cell Biol. 1984 Jan;98(1):352-7 PMID: 6707096
  5. Human anti-centromere sera recognise a 19.5 kD non-histone chromosomal protein from HeLa cells.
    Clin Exp Immunol. 1984 Oct;58(1):13-20 PMID: 6383665
  6. Controlled proteolysis of tubulin by subtilisin: localization of the site for MAP2 interaction.
    Biochemistry. 1984 Sep 25;23(20):4675-81 PMID: 6388633
  7. Identification of a family of human centromere proteins using autoimmune sera from patients with scleroderma.
    Chromosoma. 1985;91(3-4):313-21 PMID: 2579778
  8. The chromatographic determination of cystine and cysteine residues in proteins as s-beta-(4-pyridylethyl)cysteine.
    J Biol Chem. 1970 Aug 10;245(15):3868-71 PMID: 5492953
  9. Histone 3. 3. Sequence studies on the cyanogen bromide peptides; complete amino acid sequence of calf thymus histone 3.
    J Biol Chem. 1973 May 10;248(9):3261-74 PMID: 4735580
  10. Non-allelic variants of histones 2a, 2b and 3 in mammals.
    Nature. 1977 Mar 17;266(5599):273-5 PMID: 846573
  11. Nucleotide and corresponding amino acid sequences encoded by alpha and beta tubulin mRNAs.
    Nature. 1981 Feb 19;289(5799):650-5 PMID: 7464932
  12. Kinetochore structure, duplication, and distribution in mammalian cells: analysis by human autoantibodies from scleroderma patients.
    J Cell Biol. 1981 Oct;91(1):95-102 PMID: 7298727
  13. Fractionation and initial characterization of the kinetochore from mammalian metaphase chromosomes.
    J Cell Biol. 1985 Sep;101(3):1124-34 PMID: 3897244
  14. Localization of the tubulin binding site for tau protein.
    Eur J Biochem. 1985 Dec 16;153(3):595-600 PMID: 3935441
  15. Chromosomes move poleward in anaphase along stationary microtubules that coordinately disassemble from their kinetochore ends.
    J Cell Biol. 1987 Jan;104(1):9-18 PMID: 3793763
  16. A 17-kD centromere protein (CENP-A) copurifies with nucleosome core particles and with histones.
    J Cell Biol. 1987 Apr;104(4):805-15 PMID: 3558482
  17. Molecular cloning of cDNA for CENP-B, the major human centromere autoantigen.
    J Cell Biol. 1987 Apr;104(4):817-29 PMID: 2435739
  18. Mammalian kinetochore/centromere composition: a 50 kDa antigen is present in the mammalian kinetochore/centromere.
    Chromosoma. 1987;95(6):403-7 PMID: 3315496
  19. Polewards chromosome movement driven by microtubule depolymerization in vitro.
    Nature. 1988 Feb 11;331(6156):499-504 PMID: 3340202
  20. Microtubule dynamics and chromosome motion visualized in living anaphase cells.
    J Cell Biol. 1988 Apr;106(4):1185-92 PMID: 3283149
  21. Centromere proteins. I. Mitosis specific centromere antigen recognized by anti-centromere autoantibodies.
    Chromosoma. 1989 Jan;97(4):282-8 PMID: 2651051
  22. The motor for poleward chromosome movement in anaphase is in or near the kinetochore.
    J Cell Biol. 1989 Nov;109(5):2245-55 PMID: 2808528
  23. Posttranslational glutamylation of alpha-tubulin.
    Science. 1990 Jan 5;247(4938):83-5 PMID: 1967194
  24. Biochemical analysis of CENP-A, a centromeric protein with histone-like properties.
    Prog Clin Biol Res. 1989;318:61-72 PMID: 2626439
  25. CENP-B: a major human centromere protein located beneath the kinetochore.
    J Cell Biol. 1990 May;110(5):1475-88 PMID: 2335558
  26. Microinjected centromere [corrected] kinetochore antibodies interfere with chromosome movement in meiotic and mitotic mouse oocytes.
    J Cell Biol. 1990 Oct;111(4):1491-504 PMID: 2211822
  27. Injection of anticentromere antibodies in interphase disrupts events required for chromosome movement at mitosis.
    J Cell Biol. 1990 Oct;111(4):1519-33 PMID: 2211824
  28. A 47-kDa human nuclear protein recognized by antikinetochore autoimmune sera is homologous with the protein encoded by RCC1, a gene implicated in onset of chromosome condensation.
    Proc Natl Acad Sci U S A. 1990 Nov;87(21):8617-21 PMID: 2236072
  29. Establishing homologies in protein sequences.
    Methods Enzymol. 1983;91:524-45 PMID: 6855599
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.9.3734
Published
1991-05-01
Pages
3734-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51527
Subset
IM
Grants
NIGMS NIH HHS · GM15731 · United States
NIGMS NIH HHS · GM32022 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.9.3734 PMC Full Text View journal details
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