The centromere specific histone CENP-A is selectively retained in discrete foci in mammalian sperm nuclei.

Chromosoma ·Vol. 100 ·No. 1 ·1990-12-00 ·Pages 32-6

Palmer DK, O'Day K, Margolis RL

Abstract

The 17 kDa human autoantigen designated CENP-A is a centromere specific histone. We show here that CENP-A is present in tissue of bovine origin, and that it is quantitatively retained in mature spermatozoa. This result is striking, as a prominent feature of spermatogenesis in mammals is the replacement of most somatic and testes specific histones with protamines. Indirect immunofluorescence studies further show that CENP-A is retained in sperm nuclei in discrete foci, rather than being dispersed throughout the sperm head. These observations suggest that CENP-A is a functionally important component of centromeres, and that pre-existing CENP-A:DNA interactions are likely to be important in organizing the centromeres of the paternal genome during early embryogenesis.

MeSH Terms

Animals Autoantigens/analysis Cattle Cell Nucleus/chemistry Cells, Cultured Centromere/chemistry Centromere Protein A Chromosomal Proteins, Non-Histone/analysis Electrophoresis, Polyacrylamide Gel Fluorescent Antibody Technique HeLa Cells Histones/analysis Humans Immunoblotting Male Protamines Spermatozoa/chemistry Thymus Gland/chemistry,cytology Tumor Cells, Cultured

Chemicals

Autoantigens CENPA protein, human Centromere Protein A Chromosomal Proteins, Non-Histone Histones Protamines

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Palmer D K

Fred Hutchinson Cancer Research Center, Seattle, WA 98104.

O'Day K

Margolis R L

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27 references, click to expand

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Article Info

Journal

Chromosoma

Abbr.

Chromosoma

ISSN

0009-5915

DOI

10.1007/BF00337600

Published

1990-12-00

Pages

32-6

Language

English

Region

Austria

NLM ID

2985138R

Subset

External Links

PubMed source DOI: 10.1007/BF00337600 View journal details

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The centromere specific histone CENP-A is selectively retained in discrete foci in mammalian sperm nuclei.

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