Absence of mutations in marRAB or soxRS in acrB-overexpressing fluoroquinolone-resistant clinical and veterinary isolates of Escherichia coli.

Antimicrobial agents and chemotherapy ·Vol. 45 ·No. 5 ·2001-05-00 ·Pages 1550-2

Webber MA, Piddock LJ

Abstract

The amount of acrB, marA, and soxS mRNA was determined in 36 fluoroquinolone-resistant E. coli from humans and animals, 27 of which displayed a multiple-resistance phenotype. acrB mRNA was elevated in 11 of 36 strains. A mutation at codon 45 (Arg-->Cys) in acrR was found in 6 of these 11 strains. Ten of the 36 isolates appeared to overexpress soxS, and five appeared to overexpress marA. A number of mutations were found in the marR and soxR repressor genes, correlating with greater amounts of marA and soxS mRNA, respectively.

MeSH Terms

Animals Anti-Infective Agents/pharmacology Bacterial Proteins/genetics DNA-Binding Proteins/genetics Drug Resistance, Microbial/genetics Escherichia coli/drug effects,genetics Escherichia coli Proteins Fluoroquinolones Humans Microbial Sensitivity Tests Mutation Repressor Proteins/genetics Trans-Activators Transcription Factors/genetics

Chemicals

Anti-Infective Agents Bacterial Proteins DNA-Binding Proteins Escherichia coli Proteins Fluoroquinolones MarA protein, E coli MarR protein, E coli Repressor Proteins Trans-Activators Transcription Factors multiple antibiotic resistance protein B SoxR protein, Bacteria SoxS protein, E coli

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Webber M A

Antimicrobial Agents Research Group, Division of Immunity and Infection, University of Birmingham, Birmingham B15 2TT, United Kingdom.

Piddock L J

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21 references, click to expand

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Article Info

Journal

Antimicrobial agents and chemotherapy

Abbr.

Antimicrob Agents Chemother

ISSN

0066-4804

DOI

10.1128/AAC.45.5.1550-1552.2001

Published

2001-05-00

Pages

1550-2

Language

English

Region

United States

NLM ID

0315061

PMCID

PMC90504

Subset

External Links

PubMed source DOI: 10.1128/AAC.45.5.1550-1552.2001 PMC Full Text View journal details

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Absence of mutations in marRAB or soxRS in acrB-overexpressing fluoroquinolone-resistant clinical and veterinary isolates of Escherichia coli.

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