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PMID: 10991874 Published · ppublish English Journal Article

Two efflux systems expressed simultaneously in multidrug-resistant Pseudomonas aeruginosa.

Antimicrobial agents and chemotherapy ·Vol. 44 ·No. 10 ·2000-10-00 ·Pages 2861-4

Pumbwe L, Piddock LJ

Abstract

Simultaneous overexpression of MexAB-OprM and MexEF-OprN was demonstrated for a multiply antibiotic-resistant clinical isolate of Pseudomonas aeruginosa (G49). G49 also had decreased expression of OprF. No mutations in mexR or its upstream promoter region, mexT, oprM, oprF, or sigX were revealed, suggesting regulation by a hitherto undescribed locus.

MeSH Terms
Anti-Bacterial Agents/metabolism Bacterial Outer Membrane Proteins/biosynthesis,genetics Blotting, Western Carrier Proteins/biosynthesis,genetics Drug Resistance, Multiple Electrophoresis, Polyacrylamide Gel Membrane Transport Proteins Mutation/genetics Phenotype Porins/biosynthesis,genetics Promoter Regions, Genetic/genetics Pseudomonas aeruginosa/drug effects,metabolism
Chemicals
Anti-Bacterial Agents Bacterial Outer Membrane Proteins Carrier Proteins Membrane Transport Proteins OprM protein, Pseudomonas aeruginosa OprN protein, Pseudomonas aeruginosa Porins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pumbwe L
Antimicrobial Agents Research Group, Division of Immunity and Infection, University of Birmingham, Birmingham B15 2TT, United Kingdom.
Piddock L J
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.44.10.2861-2864.2000
Published
2000-10-00
Pages
2861-4
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC90165
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.44.10.2861-2864.2000 PMC Full Text View journal details
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