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PMID: 11285215 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cpx signaling pathway monitors biogenesis and affects assembly and expression of P pili.

The EMBO journal ·Vol. 20 ·No. 7 ·2001-04-02 ·Pages 1508-18

Hung DL, Raivio TL, Jones CH, Silhavy TJ, Hultgren SJ

Abstract

P pili are important virulence factors in uropathogenic Escherichia coli. The Cpx two-component signal transduction system controls a stress response and is activated by misfolded proteins in the periplasm. We have discovered new functions for the Cpx pathway, indicating that it may play a critical role in pathogenesis. P pili are assembled via the chaperone/usher pathway. Subunits that go 'OFF-pathway' during pilus biogenesis generate a signal. This signal is derived from the misfolding and aggregation of subunits that failed to come into contact with the chaperone in the periplasm. In response, Cpx not only controls the stress response, but also controls genes necessary for pilus biogenesis, and is involved in regulating the phase variation of pap expression and, potentially, the expression of a panoply of other virulence factors. This study demonstrates how the prototypic chaperone/usher pathway is intricately linked and dependent upon a signal transduction system.

MeSH Terms
Bacterial Proteins/genetics,metabolism,physiology Binding Sites Escherichia coli/genetics,metabolism,physiology Escherichia coli Proteins Fimbriae Proteins Fimbriae, Bacterial/physiology,ultrastructure Gene Expression Regulation, Bacterial Genes, Bacterial Heat-Shock Proteins/genetics,metabolism,physiology Membrane Proteins/genetics,metabolism Operon Periplasmic Proteins Protein Kinases/genetics,metabolism Serine Endopeptidases/genetics,metabolism,physiology Signal Transduction Transcription Factors/genetics
Chemicals
AtpA protein, E coli Bacterial Proteins CpxP protein, E coli CpxP protein, bacteria Escherichia coli Proteins Heat-Shock Proteins Membrane Proteins Periplasmic Proteins Transcription Factors atpD protein, E coli Fimbriae Proteins CpxR protein, Bacteria Protein Kinases CpxA protein, E coli CpxA protein, bacteria DegP protease Serine Endopeptidases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hung D L
Department of Molecular Microbiology, Box 8230, Washington University School of Medicine, 660 S. Euclid Avenue, St Louis, MO 63110, USA.
Raivio T L
Jones C H
Silhavy T J
Hultgren S J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/20.7.1508
Published
2001-04-02
Pages
1508-18
Language
English
Region
England
NLM ID
8208664
PMCID
PMC145513
Subset
IM
Grants
NIDDK NIH HHS · R01DK51406 · United States
NIAID NIH HHS · AI07172-19 · United States
NIGMS NIH HHS · R01 GM034821 · United States
NIGMS NIH HHS · GM34821 · United States
NIDDK NIH HHS · R01 DK051406 · United States
NIAID NIH HHS · T32 AI007172 · United States
NIGMS NIH HHS · R37 GM034821 · United States
External Links
PubMed source DOI: 10.1093/emboj/20.7.1508 PMC Full Text View journal details
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