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PMID: 6136465 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Genetics of digalactoside-binding adhesin from a uropathogenic Escherichia coli strain.

Infection and immunity ·Vol. 41 ·No. 3 ·1983-09-00 ·Pages 942-9

Normark S, Lark D, Hull R, Norgren M, Båga M, O'Hanley P, Schoolnik G, Falkow S

Abstract

The uropathogenic strain Escherichia coli J96 mediates mannose-resistant hemagglutination owing to production of a digalactoside-binding adhesin. A cosmid clone from this strain has been isolated that, when harbored in E. coli K-12, expressed Pap pili and this adhesin (R. Hull et al., Infect. Immun. 33:933-938, 1981). By transposon mutagenesis and by the construction of a number of hybrid plasmid derivatives, we have demonstrated that about 8.5 kilobases of DNA is required to generate a mannose-resistant hemagglutination-positive phenotype in E. coli K-12 strain P678-54. The structural gene for the Pap pili monomer, papA, has been identified and mapped close to the promotor-proximal end of the Pap operon. Although strain P678-54 that harbored a Tn5 insertion within papA showed a mannose-resistant hemagglutination-positive phenotype, it was negative in a competitive enzyme-linked immunosorbent assay with anti-Pap pilus serum. This could mean that a Pap adhesin is encoded by a region on the Pap operon that is distinct from papA.

MeSH Terms
Adhesins, Escherichia coli Animals Bacterial Proteins/analysis,genetics Chromosome Mapping DNA Transposable Elements DNA, Bacterial/genetics Escherichia coli/genetics,isolation & purification,pathogenicity Fimbriae, Bacterial/analysis Genes Genes, Bacterial Humans Mutation Nucleic Acid Hybridization Plasmids Rabbits
Chemicals
Adhesins, Escherichia coli Bacterial Proteins DNA Transposable Elements DNA, Bacterial
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Normark S
Lark D
Hull R
Norgren M
Båga M
O'Hanley P
Schoolnik G
Falkow S
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26 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.41.3.942-949.1983
Published
1983-09-00
Pages
942-9
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC264592
Subset
IM
Grants
NIAID NIH HHS · AI10885 · United States
NIAID NIH HHS · AI14740 · United States
NIAID NIH HHS · AI18462 · United States
External Links
PubMed source DOI: 10.1128/iai.41.3.942-949.1983 PMC Full Text View journal details
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