Escherichia coli pili as possible mediators of attachment to human urinary tract epithelial cells.

Infection and immunity ·Vol. 21 ·No. 1 ·1978-07-00 ·Pages 229-37

Edén CS, Hansson HA

Abstract

Presence of pili of fimbriae on Escherichia coli bacteria isolated from the urine of patients with urinary tract infection was related to the ability of the bacteria to attach to human uroepithelial cells. Piliated E. coli strains agglutinated guinea pig erythrocytes. D-Mannose and alpha-methyl-D-mannopyranoside inhibited this agglutination with all but one of the 12 strains tested. D-Mannose, D-galactose, alpha-methyl-D-mannopyranoside, and L-fucose did not afect attachment of piliated strains to uroepithelial cells. Heating as well as washing of piliated strains caused a parallel decrease of piliation and adhesive ability. Growth in glucose-enriched medium increased capsule formation but decreased piliation and adhesion. Capsulated strains retained their adhesive ability provided that pili extended outside the capsule.

MeSH Terms

Adhesiveness Culture Media Escherichia coli/growth & development,physiology,ultrastructure Fucose/pharmacology Galactose/pharmacology Hemagglutination/drug effects Humans Mannose/pharmacology Methylmannosides/pharmacology Urinary Tract/microbiology

Chemicals

Culture Media Methylmannosides Fucose Mannose Galactose

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Edén C S

Hansson H A

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25 references, click to expand

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.21.1.229-237.1978

Published

1978-07-00

Pages

229-37

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC421981

Subset

External Links

PubMed source DOI: 10.1128/iai.21.1.229-237.1978 PMC Full Text View journal details

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Escherichia coli pili as possible mediators of attachment to human urinary tract epithelial cells.

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