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PMID: 9351822 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The chaperone-assisted membrane release and folding pathway is sensed by two signal transduction systems.

The EMBO journal ·Vol. 16 ·No. 21 ·1997-11-03 ·Pages 6394-406

Jones CH, Danese PN, Pinkner JS, Silhavy TJ, Hultgren SJ

Abstract

The assembly of interactive protein subunits into extracellular structures, such as pilus fibers in the Enterobacteriaceae, is dependent on the activity of PapD-like periplasmic chaperones. The ability of PapD to undergo a beta zippering interaction with the hydrophobic C-terminus of pilus subunits facilitates their folding and release from the cytoplasmic membrane into the periplasm. In the absence of the chaperone, subunits remained tethered to the membrane and were driven off-pathway via non-productive interactions. These off-pathway reactions were detrimental to cell growth; wild-type growth was restored by co-expression of PapD. Subunit misfolding in the absence of PapD was sensed by two parallel pathways: the Cpx two-component signaling system and the sigma E modulatory pathway.

MeSH Terms
Adhesins, Escherichia coli/metabolism Bacterial Outer Membrane Proteins/metabolism Bacterial Proteins/genetics,metabolism Biological Transport Escherichia coli/genetics,metabolism Escherichia coli Proteins Fimbriae Proteins Fimbriae, Bacterial/metabolism Heat-Shock Proteins Macromolecular Substances Membrane Proteins/metabolism Models, Biological Molecular Chaperones/genetics,metabolism Periplasmic Proteins Protein Folding Protein Kinases Recombinant Fusion Proteins/metabolism Serine Endopeptidases/metabolism Sigma Factor/metabolism Signal Transduction/physiology Spheroplasts Transcription Factors/metabolism
Chemicals
Adhesins, Escherichia coli Bacterial Outer Membrane Proteins Bacterial Proteins Escherichia coli Proteins Heat-Shock Proteins Macromolecular Substances Membrane Proteins Molecular Chaperones PapD protein, E coli PapG protein, E coli Periplasmic Proteins Recombinant Fusion Proteins Sigma Factor Transcription Factors sporulation-specific sigma factors Fimbriae Proteins CpxR protein, Bacteria Protein Kinases CpxA protein, E coli CpxA protein, bacteria DegP protease Serine Endopeptidases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Jones C H
Department of Molecular Microbiology, Washington University Medical School, St Louis, MO 63110, USA.
Danese P N
Pinkner J S
Silhavy T J
Hultgren S J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/16.21.6394
Published
1997-11-03
Pages
6394-406
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1170246
Subset
IM
Grants
NIAID NIH HHS · F32AIO8665 · United States
NIGMS NIH HHS · GM07388 · United States
PHS HHS · R01A129549 · United States
External Links
PubMed source DOI: 10.1093/emboj/16.21.6394 PMC Full Text View journal details
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