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PMID: 1348692 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Conserved immunoglobulin-like features in a family of periplasmic pilus chaperones in bacteria.

The EMBO journal ·Vol. 11 ·No. 4 ·1992-04-00 ·Pages 1617-22

Holmgren A, Kuehn MJ, Brändén CI, Hultgren SJ

Abstract

Detailed structural analyses revealed a family of periplasmic chaperones in Gram-negative prokaryotes which are structurally and possibly evolutionarily related to the immunoglobulin superfamily and assist in the assembly of adhesive pili. The members of this family have similar structures consistent with the overall topology of an immunoglobulin fold. Seven pilus chaperone sequences from Escherichia coli, Haemophilus influenzae and Klebsiella pneumoniae were aligned and their consensus sequence was superimposed onto the known three-dimensional structure of PapD, a representative member of the family. The molecular details of the conserved and variable structural motifs in this family of periplasmic chaperones give important insight into their structure, function, mechanism of action and evolutionary relationship with the immunoglobulin superfamily.

MeSH Terms

Adhesins, Escherichia coli Amino Acid Sequence Bacterial Outer Membrane Proteins/chemistry,genetics Biological Evolution Escherichia coli/genetics Haemophilus influenzae/genetics Hemagglutinins/genetics Immunoglobulins/chemistry,genetics Klebsiella pneumoniae/genetics Models, Molecular Molecular Sequence Data Multigene Family Protein Conformation Sequence Homology, Nucleic Acid

Chemicals

Adhesins, Escherichia coli Bacterial Outer Membrane Proteins Hemagglutinins Immunoglobulins

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Holmgren A

Department of Molecular Biology, Biomedical Centre, Uppsala, Sweden.

Kuehn M J

Brändén C I

Hultgren S J

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23 references, click to expand

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Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1992-04-00

Pages

1617-22

Language

English

Region

England

NLM ID

8208664

PMCID

PMC556611

Subset

Grants

NIAID NIH HHS · 1R01AI29549 · United States

External Links

PubMed source PMC Full Text View journal details

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Conserved immunoglobulin-like features in a family of periplasmic pilus chaperones in bacteria.

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