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PMID: 11158529 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Monitoring the expression pattern of 1300 Arabidopsis genes under drought and cold stresses by using a full-length cDNA microarray.

The Plant cell ·Vol. 13 ·No. 1 ·2001-01-00 ·Pages 61-72

Seki M, Narusaka M, Abe H, Kasuga M, Yamaguchi-Shinozaki K, Carninci P, Hayashizaki Y, Shinozaki K

Abstract

Full-length cDNAs are essential for functional analysis of plant genes. Using the biotinylated CAP trapper method, we constructed full-length Arabidopsis cDNA libraries from plants in different conditions, such as drought-treated, cold-treated, or unstressed plants, and at various developmental stages from germination to mature seed. We prepared a cDNA microarray using approximately 1300 full-length Arabidopsis cDNAs to identify drought- and cold-inducible genes and target genes of DREB1A/CBF3, a transcription factor that controls stress-inducible gene expression. In total, 44 and 19 cDNAs for drought- and cold-inducible genes, respectively, were isolated, 30 and 10 of which were novel stress-inducible genes that have not been reported as drought- or cold-inducible genes previously. Twelve stress-inducible genes were identified as target stress-inducible genes of DREB1A, and six of them were novel. On the basis of RNA gel blot and microarray analyses, the six genes were identified as novel drought- and cold-inducible genes that are controlled by DREB1A. Eleven DREB1A target genes whose genomic sequences have been registered in the GenBank database contained the dehydration-responsive element (DRE) or DRE-related CCGAC core motif in their promoter regions. These results show that our full-length cDNA microarray is a useful material with which to analyze the expression pattern of Arabidopsis genes under drought and cold stresses, to identify target genes of stress-related transcription factors, and to identify potential cis-acting DNA elements by combining the expression data with the genomic sequence data.

MeSH Terms
Arabidopsis/genetics Arabidopsis Proteins Base Sequence Cold Temperature DNA Primers DNA, Complementary/genetics Fluorescent Dyes Gene Expression Profiling Gene Expression Regulation, Plant/physiology Nucleic Acid Hybridization Oligonucleotide Array Sequence Analysis Transcription Factors/physiology Water
Chemicals
Arabidopsis Proteins DNA Primers DNA, Complementary DREB1A protein, Arabidopsis Fluorescent Dyes Transcription Factors Water
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Seki M
Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center, 3-1-1 Koyadai, Tsukuba 305-0074, Japan.
Narusaka M
Abe H
Kasuga M
Yamaguchi-Shinozaki K
Carninci P
Hayashizaki Y
Shinozaki K
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.13.1.61
Published
2001-01-00
Pages
61-72
Language
English
Region
England
NLM ID
9208688
PMCID
PMC102214
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.13.1.61 PMC Full Text View journal details
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