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PMID: 7647294 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Promoters from kin1 and cor6.6, two homologous Arabidopsis thaliana genes: transcriptional regulation and gene expression induced by low temperature, ABA, osmoticum and dehydration.

Plant molecular biology ·Vol. 28 ·No. 4 ·1995-07-00 ·Pages 605-17

Wang H, Datla R, Georges F, Loewen M, Cutler AJ

Abstract

The Arabidopsis thaliana genes kin1 and cor6.6 belong to the same family and were expressed at higher levels following low temperature and ABA treatments. In an attempt to elucidate the mechanism of gene regulation by low temperature, the relationship between low-temperature- and abscisic acid (ABA)-induced gene expression and possible differential expression of the two genes, we have cloned a 5.3 kb genomic fragment harboring kin1 and cor6.6 and their respective 5' sequences. The putative promoters of both genes were fused to the beta-glucuronidase (GUS) coding sequence and GUS expression was analysed in transgenic tobacco and Arabidopsis plants. The cor6.6 promoter produced a higher basal level of expression than the kin1 promoter in transgenic tobacco. Enzyme assays of inducible GUS activity in transgenic Arabidopsis and tobacco plants showed that GUS activity directed by both kin1 and cor6.6 promoters was significantly induced by ABA, dehydration and osmoticum, but not by low temperature. Northern analysis revealed, in contrast, that GUS mRNA was significantly induced in these transgenic plants by low temperature. Further analysis showed that, at low temperature, GUS protein synthesis from the induced GUS mRNA was inhibited. Together these results reveal induction of kin1 and cor6.6 transcription by low temperature, exogenous ABA and dehydration. However, low-temperature expression is dramatically reduced at the translational level.

Related Genes
cor6.6 kin1
MeSH Terms
Abscisic Acid/pharmacology Antifreeze Proteins Arabidopsis/drug effects,genetics Arabidopsis Proteins Base Sequence Cloning, Molecular Cold Temperature Enzyme Induction Gene Expression Regulation, Plant Genes, Plant/genetics Genes, Reporter Glycoproteins/genetics Molecular Sequence Data Multigene Family/genetics Osmotic Pressure Phosphoproteins/genetics Plant Proteins/genetics Plants, Genetically Modified Plants, Toxic Promoter Regions, Genetic/genetics Protein Serine-Threonine Kinases/genetics RNA, Messenger/analysis Recombinant Fusion Proteins Sequence Homology, Nucleic Acid Tobacco/genetics Transformation, Genetic
Chemicals
Antifreeze Proteins Arabidopsis Proteins Glycoproteins KIN1 protein, Arabidopsis Phosphoproteins Plant Proteins RNA, Messenger Recombinant Fusion Proteins COR6.6 protein, Arabidopsis Abscisic Acid Protein Serine-Threonine Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Wang H
Plant Biotechnology Institute, National Research Council of Canada, Saskatoon.
Datla R
Georges F
Loewen M
Cutler A J
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35 references, click to expand
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00021187
Published
1995-07-00
Pages
605-17
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00021187 View journal details
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