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PMID: 16668917 Published · ppublish English Journal Article

DNA Sequence Analysis of a Complementary DNA for Cold-Regulated Arabidopsis Gene cor15 and Characterization of the COR 15 Polypeptide.

Plant physiology ·Vol. 99 ·No. 2 ·1992-06-00 ·Pages 519-25

Lin C, Thomashow MF

Abstract

Previous studies have indicated that changes in gene expression occur in Arabidopsis thaliana L. (Heyn) during cold acclimation and that certain of the cor (cold-regulated) genes encode polypeptides that share the unusual property of remaining soluble upon boiling in aqueous solution. Here, we identify a cDNA clone for a cold-regulated gene encoding one of the "boiling-stable" polypeptides, COR15. DNA sequence analysis indicated that the gene, designated cor15, encodes a 14.7-kilodalton hydrophilic polypeptide having an N-terminal amino acid sequence that closely resembles transit peptides that target proteins to the stromal compartment of chloroplasts. Immunological studies indicated that COR15 is processed in vivo and that the mature polypeptide, COR 15m, is present in the soluble fraction of chloroplasts. Possible functions of COR 15m are discussed.

Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lin C
Department of Crop and Soil Sciences, Michigan State University, East Lansing, Michigan 48824-1325.
Thomashow M F
References (23)
23 references, click to expand
  1. Cryoprotective leaf proteins.
    Biochim Biophys Acta. 1975 Dec 15;412(2):335-49 PMID: 1191683
  2. Interrelations between Environmental Factors and Freezing Resistance of Cabbage Leaves.
    Plant Physiol. 1976 Apr;57(4):553-5 PMID: 16659524
  3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  4. cDNA sequence analysis and expression of two cold-regulated genes of Arabidopsis thaliana.
    Plant Mol Biol. 1992 Jan;18(1):13-21 PMID: 1731964
  5. A cDNA-based comparison of dehydration-induced proteins (dehydrins) in barley and corn.
    Plant Mol Biol. 1989 Jul;13(1):95-108 PMID: 2562763
  6. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  7. Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins.
    J Mol Biol. 1978 Mar 25;120(1):97-120 PMID: 642007
  8. Spinach carbonic anhydrase primary structure deduced from the sequence of a cDNA clone.
    J Biol Chem. 1990 Apr 5;265(10):5414-7 PMID: 2108138
  9. Freeze/thaw-induced destabilization of the plasma membrane and the effects of cold acclimation.
    J Bioenerg Biomembr. 1989 Feb;21(1):21-41 PMID: 2651425
  10. The primary structure of spinach glycolate oxidase deduced from the DNA sequence of a cDNA clone.
    J Biol Chem. 1987 Nov 25;262(33):15825-8 PMID: 2824469
  11. Drought and freezing tolerance and adaptation in plants: some evidence of near equivalences.
    Cryobiology. 1983 Aug;20(4):487-503 PMID: 6617235
  12. Cold Resistance and Injury in Woody Plants: Knowledge of hardy plant adaptations to freezing stress may help us to reduce winter damage.
    Science. 1970 Sep 25;169(3952):1269-78 PMID: 17772511
  13. Molecular Cloning and Expression of cor (Cold-Regulated) Genes in Arabidopsis thaliana.
    Plant Physiol. 1990 Jul;93(3):1246-52 PMID: 16667586
  14. Heat-stable proteins and abscisic Acid action in barley aleurone cells.
    Plant Physiol. 1989 Dec;91(4):1520-6 PMID: 16667211
  15. Cold acclimation in Arabidopsis and wheat : a response associated with expression of related genes encoding ;boiling-stable' polypeptides.
    Plant Physiol. 1990 Nov;94(3):1078-83 PMID: 16667799
  16. Molecular cloning and relationship to freezing tolerance of cold-acclimation-specific genes of alfalfa.
    Plant Physiol. 1989 Jan;89(1):375-80 PMID: 16666542
  17. A conserved cleavage-site motif in chloroplast transit peptides.
    FEBS Lett. 1990 Feb 26;261(2):455-8 PMID: 2311769
  18. A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots.
    Anal Biochem. 1984 Jan;136(1):175-9 PMID: 6424501
  19. Use of helical wheels to represent the structures of proteins and to identify segments with helical potential.
    Biophys J. 1967 Mar;7(2):121-35 PMID: 6048867
  20. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  21. Cloning and characterization of a cold- and ABA-inducible Arabidopsis gene.
    Plant Mol Biol. 1990 Jul;15(1):137-44 PMID: 2151730
  22. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  23. Cold Acclimation in Arabidopsis thaliana.
    Plant Physiol. 1988 Jul;87(3):745-50 PMID: 16666219
Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.99.2.519
Published
1992-06-00
Pages
519-25
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC1080494
External Links
PubMed source DOI: 10.1104/pp.99.2.519 PMC Full Text View journal details
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