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PMID: 8425058 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Characterization of a spinach gene responsive to low temperature and water stress.

Plant molecular biology ·Vol. 21 ·No. 2 ·1993-01-00 ·Pages 291-305

Neven LG, Haskell DW, Hofig A, Li QB, Guy CL

Abstract

The characterization of a cDNA for an 85 kDa spinach protein, CAP85 (cold acclimation protein) that is responsive to cold acclimation and water stress is described. Both transcript and protein levels are increased during cold acclimation and water stress. A novel characteristic of CAP85 is the presence of an 11 amino acid, lysine-rich repeat, common to Group 2 LEAs (late embryogenesis abundant proteins), which is included within a larger repeating motif present in 11 copies. Two other motifs of 8 and 16 residues are also found in three and four copies, respectively. CAP85 like other dehydrins and cold-regulated polypeptides remains soluble upon boiling. Protein blot analyses indicate that CAP85 protein is expressed in all aerial tissues as well as in roots. RNA blots show the presence of mRNA for the 85 kDa protein in leaf, petiole, and root tissue. Cell fractionation studies suggest that CAP85 is predominantly found in the cytosol.

MeSH Terms
Acclimatization Amino Acid Sequence Base Sequence Cloning, Molecular Cold Temperature DNA/genetics,isolation & purification Gene Library Heat-Shock Proteins/biosynthesis,genetics,isolation & purification Molecular Sequence Data Molecular Weight Plant Proteins/biosynthesis,genetics,isolation & purification Plants/genetics Polymerase Chain Reaction Protein Biosynthesis Repetitive Sequences, Nucleic Acid Sequence Homology, Amino Acid Transcription, Genetic Water
Chemicals
CAP85 protein, Spinacia oleracea Heat-Shock Proteins Plant Proteins Water DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Neven L G
Department of Environmental Horticulture, Institute of Food and Agricultural Sciences, University of Florida, Gainesville 32611.
Haskell D W
Hofig A
Li Q B
Guy C L
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00019945
Published
1993-01-00
Pages
291-305
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
M96259
External Links
PubMed source DOI: 10.1007/BF00019945 View journal details
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