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PMID: 10810145 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Differential gene expression in response to mechanical wounding and insect feeding in Arabidopsis.

The Plant cell ·Vol. 12 ·No. 5 ·2000-05-00 ·Pages 707-20

Reymond P, Weber H, Damond M, Farmer EE

Abstract

Wounding in multicellular eukaryotes results in marked changes in gene expression that contribute to tissue defense and repair. Using a cDNA microarray technique, we analyzed the timing, dynamics, and regulation of the expression of 150 genes in mechanically wounded leaves of Arabidopsis. Temporal accumulation of a group of transcripts was correlated with the appearance of oxylipin signals of the jasmonate family. Analysis of the coronatine-insensitive coi1-1 Arabidopsis mutant that is also insensitive to jasmonate allowed us to identify a large number of COI1-dependent and COI1-independent wound-inducible genes. Water stress was found to contribute to the regulation of an unexpectedly large fraction of these genes. Comparing the results of mechanical wounding with damage by feeding larvae of the cabbage butterfly (Pieris rapae) resulted in very different transcript profiles. One gene was specifically induced by insect feeding but not by wounding; moreover, there was a relative lack of water stress-induced gene expression during insect feeding. These results help reveal a feeding strategy of P. rapae that may minimize the activation of a subset of water stress-inducible, defense-related genes.

MeSH Terms
Animals Arabidopsis/genetics,parasitology,physiology DNA, Complementary Gene Expression Regulation, Plant Insecta/physiology RNA, Messenger/genetics Signal Transduction
Chemicals
DNA, Complementary RNA, Messenger
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Reymond P
Laboratoire de Biologie et de Physiologie Végétales, Institut d'Ecologie, Université de Lausanne, Bâtiment de Biologie, 1015 Switzerland.
Weber H
Damond M
Farmer E E
References (39)
39 references, click to expand
  1. Arabidopsis thaliana responses to mechanical stimulation do not require ETR1 or EIN2.
    Plant Physiol. 1998 Feb;116(2):643-9 PMID: 9489014
  2. DNA arrays for analysis of gene expression.
    Methods Enzymol. 1999;303:179-205 PMID: 10349646
  3. Jasmonic acid-dependent and -independent signaling pathways control wound-induced gene activation in Arabidopsis thaliana.
    Plant Physiol. 1997 Oct;115(2):817-26 PMID: 9342878
  4. Regulation of synthesis of proteinase inhibitors I and II mRNAs in leaves of wounded tomato plants.
    Planta. 1986 Nov;169(3):399-405 PMID: 24232653
  5. Wound signaling in tomato plants. Evidence that aba is not a primary signal for defense gene activation
    Plant Physiol. 1998 Jun;117(2):687-93 PMID: 9625722
  6. Promoter and expression studies on an Arabidopsis thaliana dehydrin gene.
    FEBS Lett. 1996 Mar 4;381(3):252-6 PMID: 8601466
  7. A DNA microarray system for analyzing complex DNA samples using two-color fluorescent probe hybridization.
    Genome Res. 1996 Jul;6(7):639-45 PMID: 8796352
  8. Jasmonate and salicylate as global signals for defense gene expression.
    Curr Opin Plant Biol. 1998 Oct;1(5):404-11 PMID: 10066616
  9. Cluster analysis and display of genome-wide expression patterns.
    Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14863-8 PMID: 9843981
  10. The Arabidopsis NIM1 protein shows homology to the mammalian transcription factor inhibitor I kappa B.
    Plant Cell. 1997 Mar;9(3):425-39 PMID: 9090885
  11. Arabidopsis Mutants Selected for Resistance to the Phytotoxin Coronatine Are Male Sterile, Insensitive to Methyl Jasmonate, and Resistant to a Bacterial Pathogen.
    Plant Cell. 1994 May;6(5):751-759 PMID: 12244256
  12. Quantitative monitoring of gene expression patterns with a complementary DNA microarray.
    Science. 1995 Oct 20;270(5235):467-70 PMID: 7569999
  13. Jasmonate is essential for insect defense in Arabidopsis.
    Proc Natl Acad Sci U S A. 1997 May 13;94(10):5473-7 PMID: 11038546
  14. Exploiting the triple response of Arabidopsis to identify ethylene-related mutants.
    Plant Cell. 1990 Jun;2(6):513-23 PMID: 2152173
  15. Leucine aminopeptidase RNAs, proteins, and activities increase in response to water deficit, salinity, and the wound signals systemin, methyl jasmonate, and abscisic acid
    Plant Physiol. 1999 Aug;120(4):979-92 PMID: 10444081
  16. Reactive oxygen intermediates mediate a systemic signal network in the establishment of plant immunity.
    Cell. 1998 Mar 20;92(6):773-84 PMID: 9529253
  17. Evidence for Chewing Insect-Specific Molecular Events Distinct from a General Wound Response in Leaves.
    Plant Physiol. 1997 Dec;115(4):1299-1305 PMID: 12223872
  18. Jasmonic acid-dependent and -independent wound signal transduction pathways are differentially regulated by Ca2+/calmodulin in Arabidopsis thaliana.
    Mol Gen Genet. 1998 May;258(4):412-9 PMID: 9648747
  19. BIOSYNTHESIS AND ACTION OF JASMONATES IN PLANTS.
    Annu Rev Plant Physiol Plant Mol Biol. 1997 Jun;48:355-381 PMID: 15012267
  20. Cross-talk between wound signalling pathways determines local versus systemic gene expression in Arabidopsis thaliana.
    Plant J. 1999 Oct;20(2):135-142 PMID: 10571873
  21. ER5, a tomato cDNA encoding an ethylene-responsive LEA-like protein: characterization and expression in response to drought, ABA and wounding.
    Plant Mol Biol. 1997 Dec;35(6):847-54 PMID: 9426604
  22. Rain-, wind-, and touch-induced expression of calmodulin and calmodulin-related genes in Arabidopsis.
    Cell. 1990 Feb 9;60(3):357-64 PMID: 2302732
  23. Separate jasmonate-dependent and salicylate-dependent defense-response pathways in Arabidopsis are essential for resistance to distinct microbial pathogens.
    Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):15107-11 PMID: 9844023
  24. An octadecanoid pathway mutant (JL5) of tomato is compromised in signaling for defense against insect attack.
    Plant Cell. 1996 Nov;8(11):2067-77 PMID: 8953771
  25. The Arabidopsis NPR1 gene that controls systemic acquired resistance encodes a novel protein containing ankyrin repeats.
    Cell. 1997 Jan 10;88(1):57-63 PMID: 9019406
  26. Vein-cutting behavior: insect counterploy to the latex defense of plants.
    Science. 1987 Aug 21;237(4817):898-901 PMID: 3616620
  27. Receptor-mediated activation of a MAP kinase in pathogen defense of plants.
    Science. 1997 Jun 27;276(5321):2054-7 PMID: 9197271
  28. COI1: an Arabidopsis gene required for jasmonate-regulated defense and fertility.
    Science. 1998 May 15;280(5366):1091-4 PMID: 9582125
  29. Molecular cloning and characterization of 12-oxophytodienoate reductase, an enzyme of the octadecanoid signaling pathway from Arabidopsis thaliana. Structural and functional relationship to yeast old yellow enzyme.
    J Biol Chem. 1997 Oct 31;272(44):28066-72 PMID: 9346960
  30. Myelin basic protein kinase activity in tomato leaves is induced systemically by wounding and increases in response to systemin and oligosaccharide elicitors.
    Proc Natl Acad Sci U S A. 1997 Sep 30;94(20):11085-9 PMID: 9380763
  31. Reversible protein phosphorylation regulates jasmonic acid-dependent and -independent wound signal transduction pathways in Arabidopsis thaliana.
    Plant J. 1998 Jan;13(2):153-65 PMID: 9680973
  32. Regulation of a hevein-like gene in Arabidopsis.
    Mol Plant Microbe Interact. 1993 Nov-Dec;6(6):680-5 PMID: 8118053
  33. De Novo Biosynthesis of Volatiles Induced by Insect Herbivory in Cotton Plants.
    Plant Physiol. 1997 Aug;114(4):1161-1167 PMID: 12223763
  34. Ethylene as a Signal Mediating the Wound Response of Tomato Plants
    Science. 1996 Dec 13;274(5294):1914-7 PMID: 8943205
  35. Dinor-oxo-phytodienoic acid: a new hexadecanoid signal in the jasmonate family.
    Proc Natl Acad Sci U S A. 1997 Sep 16;94(19):10473-8 PMID: 9294235
  36. Induction of 12-oxo-phytodienoic acid in wounded plants and elicited plant cell cultures.
    Plant Physiol. 1997 Nov;115(3):1057-64 PMID: 9390438
  37. Fatty acid signaling in Arabidopsis.
    Planta. 1998 Oct;206(2):167-74 PMID: 9736997
  38. A nuclear gene encoding mitochondrial proline dehydrogenase, an enzyme involved in proline metabolism, is upregulated by proline but downregulated by dehydration in Arabidopsis.
    Plant Cell. 1996 Aug;8(8):1323-35 PMID: 8776899
  39. Abscisic acid is involved in the wound-induced expression of the proteinase inhibitor II gene in potato and tomato.
    Proc Natl Acad Sci U S A. 1989 Dec;86(24):9851-5 PMID: 16594093
Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.12.5.707
Published
2000-05-00
Pages
707-20
Language
English
Region
England
NLM ID
9208688
PMCID
PMC139922
Subset
IM
Corrections
CommentIn
PMID: 10810138
External Links
PubMed source DOI: 10.1105/tpc.12.5.707 PMC Full Text View journal details
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