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PMID: 11120941 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A soxRS-constitutive mutation contributing to antibiotic resistance in a clinical isolate of Salmonella enterica (Serovar typhimurium).

Antimicrobial agents and chemotherapy ·Vol. 45 ·No. 1 ·2001-01-00 ·Pages 38-43

Koutsolioutsou A, Martins EA, White DG, Levy SB, Demple B

Abstract

The soxRS regulon is activated by redox-cycling drugs such as paraquat and by nitric oxide. The >15 genes of this system provide resistance to both oxidants and multiple antibiotics. An association between clinical quinolone resistance and elevated expression of the soxRS regulon has been observed in Escherichia coli, but this association has not been explored for other enteropathogenic bacteria. Here we describe a soxRS-constitutive mutation in a clinical strain of Salmonella enterica (serovar Typhimurium) that arose with the development of resistance to quinolones during treatment. The elevated quinolone resistance in this strain derived from a point mutation in the soxR gene and could be suppressed in trans by multicopy wild-type soxRS. Multiple-antibiotic resistance was also transferred to a laboratory strain of S. enterica by introducing the cloned mutant soxR gene from the clinical strain. The results show that constitutive expression of soxRS can contribute to antibiotic resistance in clinically relevant S. enterica.

MeSH Terms
Anti-Bacterial Agents/pharmacology Anti-Infective Agents/pharmacology Blotting, Northern Chloramphenicol/pharmacology Ciprofloxacin/pharmacology Drug Resistance, Microbial/genetics Genes, Bacterial/genetics Humans Microbial Sensitivity Tests Mutation/genetics Nalidixic Acid/pharmacology Phenotype Plasmids/genetics Salmonella Infections/microbiology Salmonella enterica/drug effects,genetics Tetracyclines/pharmacology
Chemicals
Anti-Bacterial Agents Anti-Infective Agents Tetracyclines Nalidixic Acid Ciprofloxacin Chloramphenicol
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Koutsolioutsou A
Department of Cancer Cell Biology and Division of Biological Sciences, Harvard School of Public Health, Boston, Massachusetts 02115, USA.
Martins E A
White D G
Levy S B
Demple B
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.45.1.38-43.2001
Published
2001-01-00
Pages
38-43
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC90236
Subset
IM
Grants
NCI NIH HHS · R01 CA037831 · United States
NCI NIH HHS · CA37831 · United States
NIGMS NIH HHS · GM51661 · United States
External Links
PubMed source DOI: 10.1128/AAC.45.1.38-43.2001 PMC Full Text View journal details
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