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PMID: 1093174 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Interaction of Escherichia coli dnaB and dnaC(D) gene products in vitro.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 3 ·1975-03-00 ·Pages 921-5

Wickner S, Hurwitz J

Abstract

Purified E. coli dnaB and dnaC(D) gene products interact physically and functionally in vitro. This interaction was demonstrated as follows: (a) A complex of dnaB and dnaC(D) gene products was isolated by gel filtration; ATP specifically was required for isolation of the complex. (b) The DNA-independent ribonucleoside triphosphatase activity associated with dnaB gene product was inhibited by dnaC(D) gene product. (c) The dnaC(D) gene product was protected from inactivation by N-ethyl-maleimide by the combination of dnaB gene product and ATP; this protection required ATP specifically.

MeSH Terms
Adenosine Triphosphate/metabolism Bacterial Proteins/isolation & purification,metabolism Chromatography, Gel DNA Replication DNA, Bacterial/metabolism Escherichia coli/metabolism Ethylmaleimide/pharmacology Genes
Chemicals
Bacterial Proteins DNA, Bacterial Adenosine Triphosphate Ethylmaleimide
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wickner S
Hurwitz J
References (23)
23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.3.921
Published
1975-03-00
Pages
921-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432433
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.3.921 PMC Full Text View journal details
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