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PMID: 4599623 Published · ppublish English Journal Article

Studies on in vitro DNA synthesis: purification of dna C gene product containing dna D activity from Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 8 ·1973-08-00 ·Pages 2369-73

Wickner S, Berkower I, Wright M, Hurwitz J

Abstract

The conversion of varphiX174 single-stranded DNA to duplex DNA by extracts of E. coli requires products of the E. coli DNA replication genes. By use of this complementation system, the dna C gene product has been purified from wild-type E. coli as well as from a dna C temperature-sensitive mutant. The latter preparations are temperature sensitive when compared to the wild-type gene product. The dna C and dna D gene products copurify, have similar characteristics, are both temperature sensitive in preparations from dna C temperature-sensitive cells, and are both undetectable in preparations from dna D temperature-sensitive cells.

MeSH Terms
Bacterial Proteins/biosynthesis,isolation & purification,metabolism Centrifugation, Density Gradient Chromatography, DEAE-Cellulose Coliphages DNA, Bacterial/biosynthesis DNA, Viral Escherichia coli/analysis,metabolism Genes Molecular Weight Nucleotides/metabolism Templates, Genetic Thymine Nucleotides/metabolism
Chemicals
Bacterial Proteins DNA, Bacterial DNA, Viral Nucleotides Thymine Nucleotides
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wickner S
Berkower I
Wright M
Hurwitz J
References (19)
19 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.70.8.2369
Published
1973-08-00
Pages
2369-73
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC433737
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.70.8.2369 PMC Full Text View journal details
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