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PMID: 10913179 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Genetic interactions between TFIIS and the Swi-Snf chromatin-remodeling complex.

Molecular and cellular biology ·Vol. 20 ·No. 16 ·2000-08-00 ·Pages 5960-73

Davie JK, Kane CM

Abstract

The eukaryotic transcript elongation factor TFIIS enables RNA polymerase II to read through blocks to elongation in vitro and interacts genetically with a variety of components of the transcription machinery in vivo. In Saccharomyces cerevisiae, the gene encoding TFIIS (PPR2) is not essential, and disruption strains exhibit only mild phenotypes and an increased sensitivity to 6-azauracil. The nonessential nature of TFIIS encouraged the use of a synthetic lethal screen to elucidate the in vivo roles of TFIIS as well as provide more information on other factors involved in the regulation of transcript elongation. Several genes were identified that are necessary for either cell survival or robust growth when the gene encoding TFIIS has been disrupted. These include UBP3, KEX2, STT4, and SWI2/SNF2. SWI1 and SNF5 disruptions were also synthetically lethal with ppr2Delta, suggesting that the reduced ability to remodel chromatin confers the synthetic phenotype. The synthetic phenotypes show marked osmosensitivity and cytoskeletal defects, including a terminal hyperelongated bud phenotype with the Swi-Snf complex. These results suggest that genes important in osmoregulation, cell membrane synthesis and integrity, and cell division may require the Swi-Snf complex and TFIIS for efficient transcription. The detection of these genetic interactions provides another functional link between the Swi-Snf complex and the elongation machinery.

MeSH Terms
Adenosine Triphosphatases Chromatin/genetics Chromosomal Proteins, Non-Histone DNA-Binding Proteins/genetics Fungal Proteins/genetics Gene Expression Regulation, Fungal Nuclear Proteins Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Transcription Factors/genetics Transcription Factors, General Transcriptional Activation Transcriptional Elongation Factors
Chemicals
Chromatin Chromosomal Proteins, Non-Histone DNA-Binding Proteins Fungal Proteins Nuclear Proteins SWI1 protein, S cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Transcription Factors, General Transcriptional Elongation Factors transcription factor S-II Adenosine Triphosphatases SNF2 protein, S cerevisiae
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Davie J K
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3202, USA.
Kane C M
References (79)
79 references, click to expand
  1. Activator-dependent regulation of transcriptional pausing on nucleosomal templates.
    Genes Dev. 1996 Jun 15;10(12):1479-90 PMID: 8666232
  2. A new efficient gene disruption cassette for repeated use in budding yeast.
    Nucleic Acids Res. 1996 Jul 1;24(13):2519-24 PMID: 8692690
  3. Essential role of Swp73p in the function of yeast Swi/Snf complex.
    Genes Dev. 1996 Sep 1;10(17):2131-44 PMID: 8804308
  4. Life with 6000 genes.
    Science. 1996 Oct 25;274(5287):546, 563-7 PMID: 8849441
  5. RSC, an essential, abundant chromatin-remodeling complex.
    Cell. 1996 Dec 27;87(7):1249-60 PMID: 8980231
  6. Domain organization of Escherichia coli transcript cleavage factors GreA and GreB.
    J Biol Chem. 1997 Mar 14;272(11):7201-10 PMID: 9054416
  7. Restricted expression of a member of the transcription elongation factor S-II family in testicular germ cells during and after meiosis.
    J Biochem. 1997 Mar;121(3):598-603 PMID: 9133631
  8. Sfh1p, a component of a novel chromatin-remodeling complex, is required for cell cycle progression.
    Mol Cell Biol. 1997 Jun;17(6):3323-34 PMID: 9154831
  9. A review of phenotypes in Saccharomyces cerevisiae.
    Yeast. 1997 Sep 30;13(12):1099-133 PMID: 9301019
  10. Genomic characterization of a testis-specific TFIIS (TCEA2) gene.
    Genomics. 1997 Dec 15;46(3):516-9 PMID: 9441762
  11. DSIF, a novel transcription elongation factor that regulates RNA polymerase II processivity, is composed of human Spt4 and Spt5 homologs.
    Genes Dev. 1998 Feb 1;12(3):343-56 PMID: 9450929
  12. Evidence that Spt4, Spt5, and Spt6 control transcription elongation by RNA polymerase II in Saccharomyces cerevisiae.
    Genes Dev. 1998 Feb 1;12(3):357-69 PMID: 9450930
  13. Transcription elongation factor SII.
    Bioessays. 2000 Apr;22(4):327-36 PMID: 10723030
  14. Complete sequence of a eukaryotic regulatory gene.
    EMBO J. 1983;2(11):2071-3 PMID: 6139279
  15. Five SWI genes are required for expression of the HO gene in yeast.
    J Mol Biol. 1984 Oct 5;178(4):853-68 PMID: 6436497
  16. Genetic analysis of the mitotic transmission of minichromosomes.
    Cell. 1985 Feb;40(2):393-403 PMID: 3881185
  17. Molecular analysis of SNF2 and SNF5, genes required for expression of glucose-repressible genes in Saccharomyces cerevisiae.
    Mol Cell Biol. 1986 Nov;6(11):3643-51 PMID: 3540598
  18. A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains.
    Genetics. 1987 Aug;116(4):541-5 PMID: 3305158
  19. 5-Fluoroorotic acid as a selective agent in yeast molecular genetics.
    Methods Enzymol. 1987;154:164-75 PMID: 3323810
  20. Molecular cloning and characterization of cDNA for eukaryotic transcription factor S-II.
    J Biol Chem. 1988 Mar 15;263(8):3858-63 PMID: 3346229
  21. Elevated recombination rates in transcriptionally active DNA.
    Cell. 1989 Feb 24;56(4):619-30 PMID: 2645056
  22. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.
    Genetics. 1989 May;122(1):19-27 PMID: 2659436
  23. KEX2 mutations suppress RNA polymerase II mutants and alter the temperature range of yeast cell growth.
    Mol Cell Biol. 1989 Jun;9(6):2341-9 PMID: 2668732
  24. RNA polymerase II transcription termination is mediated specifically by protein binding to a CCAAT box sequence.
    Mol Cell Biol. 1989 Nov;9(11):5254-9 PMID: 2601722
  25. Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants.
    Proc Natl Acad Sci U S A. 1990 Jun;87(12):4645-9 PMID: 2162051
  26. Cloning by function: an alternative approach for identifying yeast homologs of genes from other organisms.
    Proc Natl Acad Sci U S A. 1990 Sep;87(17):6629-33 PMID: 2204059
  27. The SNF5 protein of Saccharomyces cerevisiae is a glutamine- and proline-rich transcriptional activator that affects expression of a broad spectrum of genes.
    Mol Cell Biol. 1990 Nov;10(11):5616-25 PMID: 2233708
  28. Rapid assessment of S. cerevisiae mating type by PCR.
    Trends Genet. 1990 Aug;6(8):236 PMID: 2238077
  29. The CHL 1 (CTF 1) gene product of Saccharomyces cerevisiae is important for chromosome transmission and normal cell cycle progression in G2/M.
    EMBO J. 1990 Dec;9(13):4347-58 PMID: 2265610
  30. A functional interaction between the C-terminal domain of RNA polymerase II and the negative regulator SIN1.
    Cell. 1991 Mar 22;64(6):1135-43 PMID: 2004420
  31. Getting started with yeast.
    Methods Enzymol. 1991;194:3-21 PMID: 2005794
  32. Functional interdependence of the yeast SNF2, SNF5, and SNF6 proteins in transcriptional activation.
    Proc Natl Acad Sci U S A. 1991 Apr 1;88(7):2687-91 PMID: 1901413
  33. Isolation, DNA sequence, and regulation of a Saccharomyces cerevisiae gene that encodes DNA strand transfer protein alpha.
    Mol Cell Biol. 1991 May;11(5):2576-82 PMID: 1850099
  34. SPT5, an essential gene important for normal transcription in Saccharomyces cerevisiae, encodes an acidic nuclear protein with a carboxy-terminal repeat.
    Mol Cell Biol. 1991 Jun;11(6):3009-19 PMID: 1840633
  35. The SNF2, SNF5 and SNF6 genes are required for Ty transcription in Saccharomyces cerevisiae.
    Genetics. 1991 May;128(1):69-77 PMID: 1648006
  36. Heterogeneity and tissue-specific expression of eukaryotic transcription factor S-II-related protein mRNA.
    J Biochem. 1991 May;109(5):674-7 PMID: 1917889
  37. Yeast RNA polymerase II subunit RPB9 is essential for growth at temperature extremes.
    J Biol Chem. 1991 Oct 5;266(28):19053-5 PMID: 1918023
  38. TFIIS and strand-transfer proteins.
    Nature. 1991 Oct 10;353(6344):509 PMID: 1922360
  39. Plasmid transformation of Escherichia coli and other bacteria.
    Methods Enzymol. 1991;204:63-113 PMID: 1943786
  40. Characterization of the yeast SWI1, SWI2, and SWI3 genes, which encode a global activator of transcription.
    Cell. 1992 Feb 7;68(3):573-83 PMID: 1339306
  41. 6-Azauracil inhibition of GTP biosynthesis in Saccharomyces cerevisiae.
    Curr Genet. 1992 Jul;22(1):9-11 PMID: 1611672
  42. Factor-stimulated RNA polymerase II transcribes at physiological elongation rates on naked DNA but very poorly on chromatin templates.
    J Biol Chem. 1992 Jul 5;267(19):13647-55 PMID: 1618865
  43. SPT4, SPT5 and SPT6 interactions: effects on transcription and viability in Saccharomyces cerevisiae.
    Genetics. 1992 Oct;132(2):325-36 PMID: 1330823
  44. Yeast SNF/SWI transcriptional activators and the SPT/SIN chromatin connection.
    Trends Genet. 1992 Nov;8(11):387-91 PMID: 1332230
  45. Evidence that SNF2/SWI2 and SNF5 activate transcription in yeast by altering chromatin structure.
    Genes Dev. 1992 Dec;6(12A):2288-98 PMID: 1459453
  46. Function of the S. cerevisiae DST1/PPR2 gene in transcription elongation.
    Cell. 1993 Jan 15;72(1):12 PMID: 8422673
  47. Morphogenesis in the yeast cell cycle: regulation by Cdc28 and cyclins.
    J Cell Biol. 1993 Mar;120(6):1305-20 PMID: 8449978
  48. Molecular and genetic characterization of SPT4, a gene important for transcription initiation in Saccharomyces cerevisiae.
    Mol Gen Genet. 1993 Mar;237(3):449-59 PMID: 8483459
  49. Chromatin remodeling machines: similar motors, ulterior motives.
    Trends Biochem Sci. 1998 Jan;23(1):20-5 PMID: 9478131
  50. Vaccinia NPH-I, a DExH-box ATPase, is the energy coupling factor for mRNA transcription termination.
    Genes Dev. 1998 Feb 15;12(4):538-46 PMID: 9472022
  51. FACT, a factor that facilitates transcript elongation through nucleosomes.
    Cell. 1998 Jan 9;92(1):105-16 PMID: 9489704
  52. Phosphatidylinositol-4-phosphate 5-kinase localized on the plasma membrane is essential for yeast cell morphogenesis.
    J Biol Chem. 1998 Jun 19;273(25):15779-86 PMID: 9624177
  53. MSS4, a phosphatidylinositol-4-phosphate 5-kinase required for organization of the actin cytoskeleton in Saccharomyces cerevisiae.
    J Biol Chem. 1998 Jun 19;273(25):15787-93 PMID: 9624178
  54. Yeast transcript elongation factor (TFIIS), structure and function. I: NMR structural analysis of the minimal transcriptionally active region.
    J Biol Chem. 1998 Aug 28;273(35):22589-94 PMID: 9712887
  55. Subunits of the yeast SWI/SNF complex are members of the actin-related protein (ARP) family.
    J Biol Chem. 1998 Sep 11;273(37):23641-4 PMID: 9726966
  56. Mutations in RNA polymerase II and elongation factor SII severely reduce mRNA levels in Saccharomyces cerevisiae.
    Mol Cell Biol. 1998 Oct;18(10):5771-9 PMID: 9742094
  57. Sth1p, a Saccharomyces cerevisiae Snf2p/Swi2p homolog, is an essential ATPase in RSC and differs from Snf/Swi in its interactions with histones and chromatin-associated proteins.
    Genetics. 1998 Nov;150(3):987-1005 PMID: 9799253
  58. Rapid and phosphoinositol-dependent binding of the SWI/SNF-like BAF complex to chromatin after T lymphocyte receptor signaling.
    Cell. 1998 Nov 25;95(5):625-36 PMID: 9845365
  59. Two actin-related proteins are shared functional components of the chromatin-remodeling complexes RSC and SWI/SNF.
    Mol Cell. 1998 Nov;2(5):639-51 PMID: 9844636
  60. Elongator, a multisubunit component of a novel RNA polymerase II holoenzyme for transcriptional elongation.
    Mol Cell. 1999 Jan;3(1):109-18 PMID: 10024884
  61. NELF, a multisubunit complex containing RD, cooperates with DSIF to repress RNA polymerase II elongation.
    Cell. 1999 Apr 2;97(1):41-51 PMID: 10199401
  62. A role for the yeast SWI/SNF complex in DNA replication.
    Nucleic Acids Res. 1999 May 1;27(9):2022-8 PMID: 10198436
  63. Continuous and widespread roles for the Swi-Snf complex in transcription.
    EMBO J. 1999 Apr 15;18(8):2254-64 PMID: 10205178
  64. Chromatin-modifying and -remodeling complexes.
    Curr Opin Genet Dev. 1999 Apr;9(2):148-51 PMID: 10322131
  65. The nucleosome remodeling complex, Snf/Swi, is required for the maintenance of transcription in vivo and is partially redundant with the histone acetyltransferase, Gcn5.
    EMBO J. 1999 Jun 1;18(11):3101-6 PMID: 10357821
  66. Mechanism and regulation of transcriptional elongation by RNA polymerase II.
    Curr Opin Cell Biol. 1999 Jun;11(3):342-6 PMID: 10395562
  67. The chromatin-specific transcription elongation factor FACT comprises human SPT16 and SSRP1 proteins.
    Nature. 1999 Jul 15;400(6741):284-8 PMID: 10421373
  68. A novel gene, STT4, encodes a phosphatidylinositol 4-kinase in the PKC1 protein kinase pathway of Saccharomyces cerevisiae.
    J Biol Chem. 1994 Jan 14;269(2):1166-72 PMID: 8288577
  69. Purified yeast RNA polymerase II reads through intrinsic blocks to elongation in response to the yeast TFIIS analogue, P37.
    J Biol Chem. 1994 Jan 14;269(2):936-43 PMID: 8288647
  70. A DNA minor groove-binding ligand both potentiates and arrests transcription by RNA polymerase II. Elongation factor SII enables readthrough at arrest sites.
    J Mol Biol. 1994 Feb 25;236(3):725-37 PMID: 8114090
  71. Stimulation of RNA polymerase II elongation by chromosomal protein HMG-14.
    Science. 1994 Aug 5;265(5173):796-9 PMID: 8047885
  72. Crystal structure of the GreA transcript cleavage factor from Escherichia coli.
    Nature. 1995 Feb 16;373(6515):636-40 PMID: 7854424
  73. Structure-function relationship of yeast S-II in terms of stimulation of RNA polymerase II, arrest relief, and suppression of 6-azauracil sensitivity.
    J Biol Chem. 1995 Apr 14;270(15):8991-5 PMID: 7721809
  74. The SWI-SNF complex: a chromatin remodeling machine?
    Trends Biochem Sci. 1995 Apr;20(4):143-6 PMID: 7770913
  75. Studies on the transformation of intact yeast cells by the LiAc/SS-DNA/PEG procedure.
    Yeast. 1995 Apr 15;11(4):355-60 PMID: 7785336
  76. Isolation and characterization of a cDNA encoding a new type of human transcription elongation factor S-II.
    Gene. 1995 Dec 29;167(1-2):297-302 PMID: 8566795
  77. RNA polymerase II holoenzyme contains SWI/SNF regulators involved in chromatin remodeling.
    Cell. 1996 Jan 26;84(2):235-44 PMID: 8565069
  78. Spermatocyte-specific expression of the gene for mouse testis-specific transcription elongation factor S-II.
    FEBS Lett. 1996 Apr 29;385(1-2):21-4 PMID: 8641458
  79. Evidence that Spt6p controls chromatin structure by a direct interaction with histones.
    Science. 1996 Jun 7;272(5267):1473-6 PMID: 8633238
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.20.16.5960-5973.2000
Published
2000-08-00
Pages
5960-73
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC86073
Subset
IM
Grants
NIGMS NIH HHS · GM54012 · United States
External Links
PubMed source DOI: 10.1128/MCB.20.16.5960-5973.2000 PMC Full Text View journal details
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